干旱区生态环境知识资源中心(Arid): Quantitative determination of Nε-(carboxymethyl)lysine in sterilized milk by isotope dilution UPLC-MS/MS method without derivatization and ion pair reagents

Arid

DOI	10.1016/j.foodchem.2022.132697
	Quantitative determination of Nε-(carboxymethyl)lysine in sterilized milk by isotope dilution UPLC-MS/MS method without derivatization and ion pair reagents
	Chen, Qin; Li, Ying; Dong, Lezhen; Shi, Ranran; Wu, Zhen; Liu, Lianliang; Zhang, Jingshun; Wu, Zufang; Pan, Daodong
通讯作者	Liu, LL ; Zhang, JS ; Pan, DD
来源期刊	FOOD CHEMISTRY
ISSN	0308-8146
EISSN	1873-7072
出版年	2022
卷号	385
英文摘要	This study developed a sensitive and easy method to directly analyze N epsilon-(carboxymethyl)lysine (CML) by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Acetonitrile and water were used as the binary mobile phase without ion pair reagents, while BEH Amide column was applied to detect CML. The target substance without derivatization could be well retained and separated after the pretreatment conditions were optimized. Oasis MCX solid phase extraction (SPE) cartridge showed the best recovery rate of CML 96.7 % and purification ability among the tested SPE cartridges. There was no obvious difference in pretreatment abilities with or without protein precipitation on the CML quantitative analysis. The recovery rates 97-98 % for CML were achieved by UPLC-MS/MS, as well as the detection limit and quantification limit were 0.05 mg/kg and 0.15 mg/kg sample, respectively. This method was suitable for quantifying CML content with high recovery rate and low detection and quantification limit in sterilized milk.
英文关键词	N epsilon -(carboxymethyl)lysine Sterilized milk Ultra-performance liquid chromatography tandem mass spectrometry Quantitative determination Amide column
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000793040200015
WOS关键词	GLYCATION END-PRODUCTS ; TANDEM MASS-SPECTROMETRY ; MAILLARD REACTION ; PROTEIN ; ENDPRODUCTS ; IMPACT ; FOOD
WOS类目	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392593
推荐引用方式 GB/T 7714	Chen, Qin,Li, Ying,Dong, Lezhen,et al. Quantitative determination of Nε-(carboxymethyl)lysine in sterilized milk by isotope dilution UPLC-MS/MS method without derivatization and ion pair reagents[J],2022,385.
APA	Chen, Qin.,Li, Ying.,Dong, Lezhen.,Shi, Ranran.,Wu, Zhen.,...&Pan, Daodong.(2022).Quantitative determination of Nε-(carboxymethyl)lysine in sterilized milk by isotope dilution UPLC-MS/MS method without derivatization and ion pair reagents.FOOD CHEMISTRY,385.
MLA	Chen, Qin,et al."Quantitative determination of Nε-(carboxymethyl)lysine in sterilized milk by isotope dilution UPLC-MS/MS method without derivatization and ion pair reagents".FOOD CHEMISTRY 385(2022).