干旱区生态环境知识资源中心(Arid): EXTRACTION PROCESS ASSESSMENT AND LC-MS ANALYSIS OF TWO TYROSINE KINASE INHIBITORS IN HUMAN PLASMA

Arid

DOI	10.31925/farmacia.2022.5.10
	EXTRACTION PROCESS ASSESSMENT AND LC-MS ANALYSIS OF TWO TYROSINE KINASE INHIBITORS IN HUMAN PLASMA
	Neamtu, Simona Daniela; Manda, Costel-Valentin; Boldeanu, Mihail Virgil; Rotaru, Ionela; Neamtu, Johny; Bita, Andrei; Croitoru, Octavian; Calucica, Daniela Maria
通讯作者	Manda, CV
来源期刊	FARMACIA
ISSN	0014-8237
EISSN	2065-0019
出版年	2022
卷号	70 期号:5 页码:855-860
英文摘要	The class of tyrosine kinase inhibitors (TKIs) is represented by a group of compounds which are currently used in the treatment of different types of cancer. As plasma concentration profiles of these drugs reveal a large interindividual variability, therapeutic drug monitoring (TDM) might be necessary to adjust the therapeutic protocol. With this purpose, we developed and validated a simple and accurate method for determining plasma concentrations for two TKIs. Solid phase extraction (SPE) using Oasis PRiME HLB (R) cartridges was used for plasma sample preparation procedure. The method provided clean extracts with a recovery extraction of more than 85%. Separation was achieved by high-performance liquid chromatography (HPLC), using a C18 (4.6 x 50 mm) column, with a gradient elution of ammonium formate-acetonitrile as mobile phase. Detection was performed by mass spectrometry (MS) in Single Ion Recording (SIR) mode. Intra-day and interday precision data for both analytes were 3.8 - 7.2% and 3.6 - 7.4%, respectively. Calibration curves were both linear between 2 and 400 ng/mL with a correlation coefficient higher than 0.998. The LC-MS method was used to quantify both TKIs in human plasma in routine analysis. The method was applied to TDM of ibrutinib and ruxolitinib and may help physicians for dose adjustment.
英文关键词	TKIs Human plasma SPE LC -MS
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000880417600010
WOS关键词	SIMULTANEOUS QUANTIFICATION ; DIHYDRODIOL-METABOLITE ; MS/MS APPLICATION ; RAT PLASMA ; IBRUTINIB ; VALIDATION
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392574
推荐引用方式 GB/T 7714	Neamtu, Simona Daniela,Manda, Costel-Valentin,Boldeanu, Mihail Virgil,et al. EXTRACTION PROCESS ASSESSMENT AND LC-MS ANALYSIS OF TWO TYROSINE KINASE INHIBITORS IN HUMAN PLASMA[J],2022,70(5):855-860.
APA	Neamtu, Simona Daniela.,Manda, Costel-Valentin.,Boldeanu, Mihail Virgil.,Rotaru, Ionela.,Neamtu, Johny.,...&Calucica, Daniela Maria.(2022).EXTRACTION PROCESS ASSESSMENT AND LC-MS ANALYSIS OF TWO TYROSINE KINASE INHIBITORS IN HUMAN PLASMA.FARMACIA,70(5),855-860.
MLA	Neamtu, Simona Daniela,et al."EXTRACTION PROCESS ASSESSMENT AND LC-MS ANALYSIS OF TWO TYROSINE KINASE INHIBITORS IN HUMAN PLASMA".FARMACIA 70.5(2022):855-860.