干旱区生态环境知识资源中心(Arid): Understanding aquaporin regulation defining silicon uptake and role in arsenic, antimony and germanium stress in pigeonpea (Cajanus cajan)*

Arid

DOI	10.1016/j.envpol.2021.118606
	Understanding aquaporin regulation defining silicon uptake and role in arsenic, antimony and germanium stress in pigeonpea (Cajanus cajan)*
	Mandlik, Rushil; Singla, Pankaj; Kumawat, Surbhi; Khatri, Praveen; Ansari, Waquar; Singh, Anuradha; Sharma, Yogesh; Singh, Archana; Solanke, Amol; Nadaf, Altafhusain; Sonah, Humira; Deshmukh, Rupesh
通讯作者	Deshmukh, R
来源期刊	ENVIRONMENTAL POLLUTION
ISSN	0269-7491
EISSN	1873-6424
出版年	2022
卷号	294
英文摘要	Understanding of aquaporins (AQPs) facilitating the transport of water and many other small solutes including metalloids like silicon (Si) and arsenic (As) is important to develop stress tolerant cultivars. In the present study, 40 AQPs were identified in the genome of pigeonpea (Cajanus cajan), a pulse crop widely grown in semi-arid region and areas known to affected with heavy metals like As. Conserved domains, variation at NPA motifs, aromatic/arginine (ar/R) selectivity filters, and pore morphology defined here will be crucial in predicting solute specificity of pigeonpea AQPs. The study identified CcNIP2-1 as an AQP predicted to transporter Si (beneficial element) as well as As (hazardous element). Further Si quantification in different tissues showed about 1.66% Si in leaves which confirmed the predictions. Furthermore, scanning electron microscopy showed a higher level of Si accumulation in trichomes on the leaf surface. A significant alleviation in level of As, Sb and Ge stress was also observed when these heavy metals were supplemented with Si. Estimation of relative water content, H2O2, lipid peroxidation, proline, total chlorophyll content and other physiological parameters suggested Si derived stress tolerance. Extensive transcriptome profiling under different developmental stages from germination to senescence was performed to understand the tissue-specific regulation of different AQPs. For instance, high expression of TIP3s was observed only in reproductive tissues. Co-expression network developed using transcriptome data from 30 different conditions and tissues, showed interdependency of AQPs. Expression profiling of pigeonpea performed using real time PCR showed differential expression of AQPs after Si supplementation. The information generated about the phylogeny, distribution, molecular evolution, solute specificity, and gene expression dynamics in article will be helpful to better understand the AQP transport system in pigeonpea and other legumes.
英文关键词	Aquaporin Molecular dynamics simulations Pigeonpea Silicon localization Silicon uptake Transcriptome
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000758385600004
WOS关键词	PLASMA-MEMBRANE AQUAPORINS ; OXIDATIVE STRESS ; ARABIDOPSIS ; TRANSPORTER ; FACILITATE ; TOXICITY ; PROTEINS ; INSIGHTS ; REVEALS
WOS类目	Environmental Sciences
WOS研究方向	Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392487
推荐引用方式 GB/T 7714	Mandlik, Rushil,Singla, Pankaj,Kumawat, Surbhi,et al. Understanding aquaporin regulation defining silicon uptake and role in arsenic, antimony and germanium stress in pigeonpea (Cajanus cajan)*[J],2022,294.
APA	Mandlik, Rushil.,Singla, Pankaj.,Kumawat, Surbhi.,Khatri, Praveen.,Ansari, Waquar.,...&Deshmukh, Rupesh.(2022).Understanding aquaporin regulation defining silicon uptake and role in arsenic, antimony and germanium stress in pigeonpea (Cajanus cajan)*.ENVIRONMENTAL POLLUTION,294.
MLA	Mandlik, Rushil,et al."Understanding aquaporin regulation defining silicon uptake and role in arsenic, antimony and germanium stress in pigeonpea (Cajanus cajan)*".ENVIRONMENTAL POLLUTION 294(2022).