干旱区生态环境知识资源中心(Arid): Metagenomic binning and assembled genome analysis revealed the distinct composition of resistome and mobilome in the Ili River

Arid

DOI	10.1016/j.ecoenv.2022.113886
	Metagenomic binning and assembled genome analysis revealed the distinct composition of resistome and mobilome in the Ili River
	Song, Wenjuan; Chen, Haiyang; Xue, Nana; Wang, Shuzhi; Yang, Yuyi
通讯作者	Yang, YY
来源期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
EISSN	1090-2414
出版年	2022
卷号	242
英文摘要	Rivers play an important role in receiving and transporting the resistome among different environmental com-partments. However, the difference in resistome and mobilome between the water and sediment and their un-derlying mechanisms were still poorly understood. In this study, the Ili River, an important water source in the arid area of Central Asia, was selected as the studied target. The comprehensive profile of resistome and mobilome and their host in water and sediment were studied based on metagenomic binning and assembled genome (MAG) analysis. The relative abundance of resistome and mobilome in sediment were 28.0 - 67.8 x /Gb and 46.5 - 121.1 x /Gb, respectively, which were significantly higher than those in water (23.1 - 52.8 x/Gb and 25.3 - 67.7 x/Gb). Multidrug and macrolides-lincosamides-streptogramin (MLS) resistance genes were the main ARG types in both water and sediment from relative abundance. Transposases dominated the relative abundance of mobilome, followed by insert elements and integrases. Strong correlations were found between the relative abundance of resistome and mobilome (r > 0.6 and p < 0.01) in both water and sediment, indicating the mobilome played an important role in the propagation of resistome in the Ili River. The main hosts for multidrug resistance genes via MAG analysis differed in water (Alphaproteobacteria and Gammaproteobacteria) and sediment (Gammaproteobacteria). Distinct compositions of resistome and mobilome existed between water and sediment in the Ili River. Specificity-occupancy analysis of the differential resistome and mobilome showed that occurrence frequencies and habitat selections of the differential ARGs shaped the resistome of water and sediment. In contrast, habitat was the main driver that shaped the mobilome in the Ili River.
英文关键词	Ili River Multidrug resistance genes Resistome Mobilome Specificity-occupancy plot
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000863995600002
WOS关键词	ANTIBIOTIC-RESISTANCE GENES ; WASTE-WATER ; TREATMENT-PLANT ; SURFACE-WATER ; LAKE ; PREVALENCE ; INTEGRONS ; ABUNDANCE ; SEDIMENTS ; HOSTS
WOS类目	Environmental Sciences ; Toxicology
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392389
推荐引用方式 GB/T 7714	Song, Wenjuan,Chen, Haiyang,Xue, Nana,et al. Metagenomic binning and assembled genome analysis revealed the distinct composition of resistome and mobilome in the Ili River[J],2022,242.
APA	Song, Wenjuan,Chen, Haiyang,Xue, Nana,Wang, Shuzhi,&Yang, Yuyi.(2022).Metagenomic binning and assembled genome analysis revealed the distinct composition of resistome and mobilome in the Ili River.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,242.
MLA	Song, Wenjuan,et al."Metagenomic binning and assembled genome analysis revealed the distinct composition of resistome and mobilome in the Ili River".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 242(2022).