干旱区生态环境知识资源中心(Arid): Comparative Plastome Analyses of Ephedra przewalskii and E. monosperma (Ephedraceae)

Arid

DOI	10.3390/d14100792
	Comparative Plastome Analyses of Ephedra przewalskii and E. monosperma (Ephedraceae)
	Han, Shuang; Xia, Mingze; Yu, Jingya; Xu, Hao; Han, Yun; Zhang, Faqi
通讯作者	Zhang, FQ
来源期刊	DIVERSITY-BASEL
EISSN	1424-2818
出版年	2022
卷号	14 期号:10
英文摘要	Ephedra species were erect, branching shrubs found in desert or arid regions worldwide as the source of ephedrine alkaloids. In this study, the complete chloroplast genome of Ephedra przewalskii and E. monosperma on the Qinghai-Tibet Plateau were sequenced, assembled, and annotated. Compared with the other four published Ephedra species, the chloroplast genomes of Ephedra species were highly conservative, with a quadripartite structure. The length of the chloroplast genome was 109,569 bp in E. przewalskii with 36.6% GC and 109,604 bp in E. monosperma with 36.6% GC. We detected 118 genes in both Ephedra species, including 73 PCGs, 37 tRNA genes, and eight rRNA genes. Among them, the ndh family genes were lost, which could be used to study the phylogeny and genetic diversity of the genus Ephedra, combined with multiple highly variable intergenic spacer (IGS) regions. Codon usage preference of Ephedra species was weak. The ratio of non-synonymous substitutions and synonymous substitutions was low, showing that the PCGs of Ephedra may be under the pressure of purifying selection. ML and BI analysis showed similar phylogenetic topologies. Ephedra species clustered together in a well-supported monophyletic clade. E. przewalskii and E. monosperma were not gathered in one clade, consistent with the classification system by Flora of China. This study reveals differences in the chloroplast genomes of Ephedra, providing valuable and abundant data for the phylogenetic analysis and species identification of Ephedra.
英文关键词	Ephedra chloroplast genome phylogeny synonymous substitutions Qinghai-Tibet Plateau
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000872667000001
WOS关键词	CHLOROPLAST GENOME ; PHYLOGENETIC ANALYSIS ; DNA-SEQUENCE ; NDH GENES ; RNA ; NUCLEAR ; GNETALES ; IDENTIFICATION ; DIVERSITY ; APIACEAE
WOS类目	Biodiversity Conservation ; Ecology
WOS研究方向	Biodiversity & Conservation ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392248
推荐引用方式 GB/T 7714	Han, Shuang,Xia, Mingze,Yu, Jingya,et al. Comparative Plastome Analyses of Ephedra przewalskii and E. monosperma (Ephedraceae)[J],2022,14(10).
APA	Han, Shuang,Xia, Mingze,Yu, Jingya,Xu, Hao,Han, Yun,&Zhang, Faqi.(2022).Comparative Plastome Analyses of Ephedra przewalskii and E. monosperma (Ephedraceae).DIVERSITY-BASEL,14(10).
MLA	Han, Shuang,et al."Comparative Plastome Analyses of Ephedra przewalskii and E. monosperma (Ephedraceae)".DIVERSITY-BASEL 14.10(2022).