干旱区生态环境知识资源中心(Arid): Characterizing Crustose Lichen Communities-DNA Metabarcoding Reveals More than Meets the Eye

Arid

DOI	10.3390/d14090766
	Characterizing Crustose Lichen Communities-DNA Metabarcoding Reveals More than Meets the Eye
	Henrie, Jacob R.; Thomson, Brenden M.; Yungfleisch, Andrew August; Kerr, Michael; Leavitt, Steven D.
通讯作者	Leavitt, SD
来源期刊	DIVERSITY-BASEL
EISSN	1424-2818
出版年	2022
卷号	14 期号:9
英文摘要	Biodiversity inventories are important for informing land management strategies, conservation efforts, and for biomonitoring studies. For many organismal groups, including lichens, comprehensive, accurate inventories are challenging due to the necessity of taxonomic expertise, limitations in sampling protocols, and the commonplace occurrence of morphologically cryptic species and other undescribed species. Lichen communities in arid regions are often dominated by crustose lichens, which have been particularly difficult to incorporate into biodiversity inventories. Here, we explore the utility of DNA metabarcoding for characterizing the diversity of lichen-forming fungi at a typical crustose lichen-dominated site on the Colorado Plateau in the southwestern USA. We assessed the consistency of independent sampling efforts to comprehensively document lichen diversity, evaluated the capability of minimally trained technicians to effectively sample the lichen communities, and provide a metagenomic-based inventory of lichen diversity, including representative sequence data, for a diverse, crustose-dominate lichen community on the Colorado Plateau. Our results revealed that crustose lichen communities in the southwestern USA are more diverse than traditionally thought, and community metabarcoding is a promising strategy for characterizing the lichen-forming fungal diversity more thoroughly than other methods. However, consistently sampling the diversity of crustose lichen communities, even at small spatial scales, remains difficult. Interpreting these results within a traditional taxonomic context remains challenging without the use of vouchers.
英文关键词	amplicon sequencing biodiversity biomonitoring ecological sampling Illumina internal transcribed spacer region (ITS) inventory ITS2 semi-arid
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000858120900001
WOS关键词	BIODIVERSITY
WOS类目	Biodiversity Conservation ; Ecology
WOS研究方向	Biodiversity & Conservation ; Environmental Sciences & Ecology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392247
推荐引用方式 GB/T 7714	Henrie, Jacob R.,Thomson, Brenden M.,Yungfleisch, Andrew August,et al. Characterizing Crustose Lichen Communities-DNA Metabarcoding Reveals More than Meets the Eye[J],2022,14(9).
APA	Henrie, Jacob R.,Thomson, Brenden M.,Yungfleisch, Andrew August,Kerr, Michael,&Leavitt, Steven D..(2022).Characterizing Crustose Lichen Communities-DNA Metabarcoding Reveals More than Meets the Eye.DIVERSITY-BASEL,14(9).
MLA	Henrie, Jacob R.,et al."Characterizing Crustose Lichen Communities-DNA Metabarcoding Reveals More than Meets the Eye".DIVERSITY-BASEL 14.9(2022).