干旱区生态环境知识资源中心(Arid): Biochemical Characterization of Rare and Threatened Local Populations of Peas (Pisum sativum L.) Cultivated in the Arid Region of Southern Tunisia

Arid

DOI	10.1002/cbdv.202200595
	Biochemical Characterization of Rare and Threatened Local Populations of Peas (Pisum sativum L.) Cultivated in the Arid Region of Southern Tunisia
	Mohamed, Amina; Tlahig, Samir; Moussa, Jamila Yehmed; Ben Yahia, Leila; Bouhamda, Talel; Loumerem, Mohamed
通讯作者	Mohamed, A
来源期刊	CHEMISTRY & BIODIVERSITY
ISSN	1612-1872
EISSN	1612-1880
出版年	2022
卷号	19 期号:12
英文摘要	Pea (Pisum sativum L.) is an important legume for human and animal consumption, cultivated in many temperate regions of the world. In the present investigation, the seeds of twelve pea accessions collected from the arid regions of southern Tunisia, characterized by different colors of flowers and seed coats, were evaluated for their proteins, phenolic compounds and antioxidant activities. The protein content determined by the Bradford method ranged from 46.91 to 151.08 mg/g DW. The content of total phenol, total flavonoid and condensed tannin, ranging from 36.85 to 102.52 mg GAE/100 g DW, 6.95 to 48.11 mg QE/100 g DW, and 12.37 to 70.43 mg CE/100 g DW, respectively. These parameters were characterized respectively by the following three methods, the Folin-Ciocalteu reagent, the aluminum chloride and the vanillin assay. The LC-ESI/MS analysis identified the presence of 8 phenolic acids and 9 flavonoids which quinic acid, protocatechuic acid, hyperoside, and quercetin-3-O-rhamnosidewere detected as the most abundant compounds. Moreover, the antioxidant activity of pea seeds was evaluated by the ABTS+and DPPH tests. Indeed, DPPH and ABTS anti-free radical activity values ranged from 1.92 to 14.11 mu moL TEAC/g DW and 2.3 to 14.95 mu moL TEAC/g DW, respectively. The variability across the assessed traits revealed significant differences as computed by ANOVA tests. Else, the results showed significant correlations between seed coat color, seed shape, flower color and phenolic compounds. Therefore, genotypes characterized by a purple flower, brown seed coat, and wrinkled form had the highest levels of phenolic compounds and antioxidant activity. Correlation analysis revealed that phenolic compounds presented a strong positive correlation with antioxidant activities, while being negatively correlated with protein content. UPGMA cluster and Principal component analysis (PCA) revealed two main groups. This work highlights the nutritional value of P. sativum seeds as a good source of natural antioxidant compounds that may be useful in the food and pharmaceutical industries.
英文关键词	Pisum sativum protein phenolics flavonoids tannin DPPH ABTS
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000879917500001
WOS关键词	ANTIOXIDANT ACTIVITY ; PHENOLIC-COMPOUNDS ; POLYGONUM-EQUISETIFORME ; BIOACTIVE PROPERTIES ; PROTEIN-CONTENT ; SEED COAT ; FIELD ; IDENTIFICATION ; ENVIRONMENT ; VARIETIES
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392132
推荐引用方式 GB/T 7714	Mohamed, Amina,Tlahig, Samir,Moussa, Jamila Yehmed,et al. Biochemical Characterization of Rare and Threatened Local Populations of Peas (Pisum sativum L.) Cultivated in the Arid Region of Southern Tunisia[J],2022,19(12).
APA	Mohamed, Amina,Tlahig, Samir,Moussa, Jamila Yehmed,Ben Yahia, Leila,Bouhamda, Talel,&Loumerem, Mohamed.(2022).Biochemical Characterization of Rare and Threatened Local Populations of Peas (Pisum sativum L.) Cultivated in the Arid Region of Southern Tunisia.CHEMISTRY & BIODIVERSITY,19(12).
MLA	Mohamed, Amina,et al."Biochemical Characterization of Rare and Threatened Local Populations of Peas (Pisum sativum L.) Cultivated in the Arid Region of Southern Tunisia".CHEMISTRY & BIODIVERSITY 19.12(2022).