干旱区生态环境知识资源中心(Arid): Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions

Arid

DOI	10.3390/catal12070769
	Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions
	Balla, Amel; Silini, Allaoua; Cherif-Silini, Hafsa; Bouket, Ali Chenari; Boudechicha, Amel; Luptakova, Lenka; Alenezi, Faizah N.; Belbahri, Lassaad
通讯作者	Belbahri, L
来源期刊	CATALYSTS
EISSN	2073-4344
出版年	2022
卷号	12 期号:7
英文摘要	Cellulose represents the most abundant component of plant biomass on earth; it is degraded by cellulases, specific enzymes produced by microorganisms. However, cellulases of bacterial origin attract more interest due to their natural diversity and ability to inhabit a variety of niches, allowing the selection of cellulolytic strains resistant to environmental stresses. The screening of the cellulolytic activity of 398 bacteria isolated from various ecosystems in Algeria (cave, ruins, chott, thermal station, and rhizosphere of arid and semi-arid regions) was performed by the appearance of a hydrolysis zone on carboxymethylcellulose (CMC) medium. The cellulase activity on CMC (1%) broth allowed to select 26 strains among which 12 had the best activity (0.3 U/mL to 2.2 U/mL). Optimization of physicochemical parameters (salinity: 0-1 M NaCl; pH: 3, 4, 7, 9, and 11; temperature: 30, 45, and 50 degrees C; PEG(8000): 0 and 30%) involved in growth and cellulose production showed that the majority of strains were mesophilic, neutrophilic, or alkali- tolerant and tolerant to 30% of PEG(8000). The cellulase activity and stability under different stress allowed to retain five strains, which the most efficient. Based on the 16S-rRNA sequencing results, they belonged to the genus Bacillus. The physicochemical properties of cellulases (crude extract) showed a CMCase active over a wide range of pH (4 to 11), optimal at 50 degrees C and 60 degrees C. The inhibiting salinity effect on the activity was not detected and was negligible on the enzymatic stability. The residual CMCase activity remained between 40 and 70% in a temperature range between 40 and 70 degrees C, was stable over a wide range of saline concentrations (0-2000 mM), and was weakly affected at 30% of PEG(8000). The crude enzyme extract was able to hydrolyze both soluble and insoluble cellulosic substrates. The evaluation of the hydrolysis capacity of lignocellulosic waste revealed the ability of tested strains to degrade wheat bran, barley bran, and corncob. In addition, the enzyme showed significant multi-stress resistance on solid and liquid media. By these characteristics, these cellulolytic bacteria could be attractive to be used in various industrial and biotechnology applications.
英文关键词	cellulolytic bacteria carboxymethylcellulose cellulase activity enzymatic stability multi-stress tolerance
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000832377700001
WOS关键词	PURIFICATION ; ENZYMES ; GROWTH ; WHEAT
WOS类目	Chemistry, Physical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392062
推荐引用方式 GB/T 7714	Balla, Amel,Silini, Allaoua,Cherif-Silini, Hafsa,et al. Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions[J],2022,12(7).
APA	Balla, Amel.,Silini, Allaoua.,Cherif-Silini, Hafsa.,Bouket, Ali Chenari.,Boudechicha, Amel.,...&Belbahri, Lassaad.(2022).Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions.CATALYSTS,12(7).
MLA	Balla, Amel,et al."Screening of Cellulolytic Bacteria from Various Ecosystems and Their Cellulases Production under Multi-Stress Conditions".CATALYSTS 12.7(2022).