干旱区生态环境知识资源中心(Arid): Identification of the key flavonoid and lipid synthesis proteins in the pulp of two sea buckthorn cultivars at different developmental stages

Arid

DOI	10.1186/s12870-022-03688-5
	Identification of the key flavonoid and lipid synthesis proteins in the pulp of two sea buckthorn cultivars at different developmental stages
	Du, Wei; Ding, Jian; Lu, Shunguang; Wen, Xiufeng; Hu, Jianzhong; Ruan, Chengjiang
通讯作者	Ruan, CJ
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2022
卷号	22 期号:1
英文摘要	Background Sea buckthorn is an economically important woody plant for desertification control and water soil conservation. Its berry pulp is rich in flavonoids and unsaturated fatty acids. Cultivars containing high oil and flavonoid contents have higher economic value and will increase in the planting area. However, the cause of the differences in oil and flavonoid contents among cultivars is still unclear. The influence of key enzymes in the lipid and flavonoid synthesis pathways on their content needs to be explored and clarified. Results The flavonoid content in XE (Xin'e 3) was 54% higher than that in SJ (Suiji 1). Rutin was the main flavonoid in sea buckthorn pulp, and the differences in the rutin content could cause flavonoid differences between the two cultivars. The oil content of XE was 31.58% higher than that of SJ, and the difference in oil content was highest at 50-70 DAF. High-throughput proteomics was used to quantify key enzymes of flavonoid and lipid synthesis pathways in two cultivars at three developmental stages. By functional annotation and KEGG analysis, 41 key enzymes related to phenylpropanoid biosynthesis, flavonoid biosynthesis, flavone and flavonol biosynthesis, fatty acid biosynthesis and TAG biosynthesis were quantified. CHS, F3H, ANS, fabD, FATA, FAB2, LPIN and plcC showed significant differences between the two cultivars. In addition, we quantified 6 oleosins. With the exception of a 16 kDa oleosin, the other oleosins in the two cultivars were positively correlated with oil content. Conclusions In the flavonoid synthesis pathway, CHS and F3H were the main enzymes responsible for the difference in flavonoid content between the two cultivars. In the lipid synthesis pathway, LPIN, plcC and MGD were the main enzymes with different contents in the middle to late stages. Higher contents of LPIN and plcC in XE than in SJ could cause DAG to generate TAG from PC, since the difference in DGAT between the two cultivars was not significant. Investigating the causes of flavonoid and oil content differences among different cultivars from the perspective of proteomics, could provide a basis for understanding the regulatory mechanism of flavonoids and lipid synthesis in sea buckthorn pulp.
英文关键词	Sea buckthorn TAG biosynthesis Flavonoid biosynthesis Cultivar variation Proteomics
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000812259600002
WOS关键词	EXPRESSION ANALYSIS ; FATTY-ACIDS ; SEED OIL ; BIOSYNTHESIS ; ACCUMULATION ; SYNTHASE ; GENES ; PROTEOMICS ; BERRIES ; PATHWAY
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392010
推荐引用方式 GB/T 7714	Du, Wei,Ding, Jian,Lu, Shunguang,et al. Identification of the key flavonoid and lipid synthesis proteins in the pulp of two sea buckthorn cultivars at different developmental stages[J],2022,22(1).
APA	Du, Wei,Ding, Jian,Lu, Shunguang,Wen, Xiufeng,Hu, Jianzhong,&Ruan, Chengjiang.(2022).Identification of the key flavonoid and lipid synthesis proteins in the pulp of two sea buckthorn cultivars at different developmental stages.BMC PLANT BIOLOGY,22(1).
MLA	Du, Wei,et al."Identification of the key flavonoid and lipid synthesis proteins in the pulp of two sea buckthorn cultivars at different developmental stages".BMC PLANT BIOLOGY 22.1(2022).