干旱区生态环境知识资源中心(Arid): Global analysis of switchgrass (Panicum virgatum L.) transcriptomes in response to interactive effects of drought and heat stresses

Arid

DOI	10.1186/s12870-022-03477-0
	Global analysis of switchgrass (Panicum virgatum L.) transcriptomes in response to interactive effects of drought and heat stresses
	Hayford, Rita K.; Serba, Desalegn D.; Xie, Shaojun; Ayyappan, Vasudevan; Thimmapuram, Jyothi; Saha, Malay C.; Wu, Cathy H.; Kalavacharla, Venu Kal
通讯作者	Kalavacharla, VK
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2022
卷号	22 期号:1
英文摘要	Background Sustainable production of high-quality feedstock has been of great interest in bioenergy research. Despite the economic importance, high temperatures and water deficit are limiting factors for the successful cultivation of switchgrass in semi-arid areas. There are limited reports on the molecular basis of combined abiotic stress tolerance in switchgrass, particularly the combination of drought and heat stress. We used transcriptomic approaches to elucidate the changes in the response of switchgrass to drought and high temperature simultaneously. Results We conducted solely drought treatment in switchgrass plant Alamo AP13 by withholding water after 45 days of growing. For the combination of drought and heat effect, heat treatment (35 degrees C/25 degrees C day/night) was imposed after 72 h of the initiation of drought. Samples were collected at 0 h, 72 h, 96 h, 120 h, 144 h, and 168 h after treatment imposition, total RNA was extracted, and RNA-Seq conducted. Out of a total of 32,190 genes, we identified 3912, as drought (DT) responsive genes, 2339 and 4635 as, heat (HT) and drought and heat (DTHT) responsive genes, respectively. There were 209, 106, and 220 transcription factors (TFs) differentially expressed under DT, HT and DTHT respectively. Gene ontology annotation identified the metabolic process as the significant term enriched in DTHT genes. Other biological processes identified in DTHT responsive genes included: response to water, photosynthesis, oxidation-reduction processes, and response to stress. KEGG pathway enrichment analysis on DT and DTHT responsive genes revealed that TFs and genes controlling phenylpropanoid pathways were important for individual as well as combined stress response. For example, hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT) from the phenylpropanoid pathway was induced by single DT and combinations of DTHT stress. Conclusion Through RNA-Seq analysis, we have identified unique and overlapping genes in response to DT and combined DTHT stress in switchgrass. The combination of DT and HT stress may affect the photosynthetic machinery and phenylpropanoid pathway of switchgrass which negatively impacts lignin synthesis and biomass production of switchgrass. The biological function of genes identified particularly in response to DTHT stress could further be confirmed by techniques such as single point mutation or RNAi.
英文关键词	Panicum virgatum Transcriptome Drought stress Heat stress Transcription factors Gene ontology Differential gene expression
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000766186700001
WOS关键词	ABSCISIC-ACID ; PLANT-RESPONSES ; GENE-EXPRESSION ; ABIOTIC STRESS ; ENVIRONMENTAL-STRESSES ; TOLERANCE ; SALINITY ; RESISTANCE ; YIELD ; SHOCK
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/392007
推荐引用方式 GB/T 7714	Hayford, Rita K.,Serba, Desalegn D.,Xie, Shaojun,et al. Global analysis of switchgrass (Panicum virgatum L.) transcriptomes in response to interactive effects of drought and heat stresses[J],2022,22(1).
APA	Hayford, Rita K..,Serba, Desalegn D..,Xie, Shaojun.,Ayyappan, Vasudevan.,Thimmapuram, Jyothi.,...&Kalavacharla, Venu Kal.(2022).Global analysis of switchgrass (Panicum virgatum L.) transcriptomes in response to interactive effects of drought and heat stresses.BMC PLANT BIOLOGY,22(1).
MLA	Hayford, Rita K.,et al."Global analysis of switchgrass (Panicum virgatum L.) transcriptomes in response to interactive effects of drought and heat stresses".BMC PLANT BIOLOGY 22.1(2022).