干旱区生态环境知识资源中心(Arid): Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica)

Arid

DOI	10.3390/biology11081143
	Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica)
	Akulava, Volha; Miamin, Uladzislau; Akhremchuk, Katsiaryna; Valentovich, Leonid; Dolgikh, Andrey; Shapaval, Volha
通讯作者	Akulava, V
来源期刊	BIOLOGY-BASEL
EISSN	2079-7737
出版年	2022
卷号	11 期号:8
英文摘要	Simple Summary The characterization of microbial communities from Antarctic temporary meltwater ponds is limited, while they could serve as a source of biotechnologically interesting microorganisms. In this study, we characterized a set of bacteria isolated from the sea-affected temporary meltwater ponds in the East Antarctica area of the Vecherny region of the Thala Hills Oasis, Enderby Land. The isolated meltwater bacteria were identified as Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes, where Proteobacteria and Actinobacteria were predominant. The isolated bacteria were able to grow in a relatively wide temperature range between 4 degrees C and 37 degrees C, with an optimal temperature range of 18-25 degrees C. Further, most of the isolates showed an ability to secrete lipases and proteases, and several of them were pigmented. Bacterial isolates from the genera Pseudomonas and Acinetobacter exhibited multi-resistance against beta-lactams, sulfonamide, macrolide, diaminopyrimidines, and chloramphenicol antibiotics. This study shows that bacterial communities from the temporary meltwater ponds in East Antarctica consist of metabolically versatile bacteria that might be defined by their location near the sea and the close presence of animals, penguins and skuas in particular. In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (-67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. Water samples from the studied MPs showed alkaline pH (from 8.0 to 10.1) and highly varied total dissolved solids (86-94,000 mg/L). In total, twenty-nine bacterial isolates were retrieved from the studied MPs. The phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolated bacteria belong to the phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes and the twelve genera Pseudomonas, Shewanella, Acinetobacter, Sporosarcina, Facklamia, Carnobacterium, Arthrobacter, Brachybacterium, Micrococcus, Agrococcus, Leifsonia, and Flavobacterium. Most of the isolated bacteria were psychrotrophs and showed the production of one or more extracellular enzymes. Lipolytic and proteolytic activities were more prevalent among the isolates. Five isolates from the Actinobacteria phylum and one isolate from the Bacteroidetes phylum had strong pigmentation. Antibiotic susceptibility testing revealed that most of the isolates are resistant to at least one antibiotic, and seven isolates showed multi-resistance.
英文关键词	meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity antibiotic resistance
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000847009500001
WOS关键词	MCMURDO ICE SHELF ; SP-NOV ; PSYCHROTOLERANT BACTERIUM ; PSYCHROTROPHIC BACTERIA ; PSYCHROPHILIC BACTERIA ; MARINE-BACTERIA ; BRATINA ISLAND ; DIVERSITY ; LAKE ; COMMUNITIES
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/391984
推荐引用方式 GB/T 7714	Akulava, Volha,Miamin, Uladzislau,Akhremchuk, Katsiaryna,et al. Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica)[J],2022,11(8).
APA	Akulava, Volha,Miamin, Uladzislau,Akhremchuk, Katsiaryna,Valentovich, Leonid,Dolgikh, Andrey,&Shapaval, Volha.(2022).Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica).BIOLOGY-BASEL,11(8).
MLA	Akulava, Volha,et al."Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica)".BIOLOGY-BASEL 11.8(2022).