Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.4149/av_2022_409 |
Genome sequence of a Papaya ringspot virus from khejri (Prosopis cineraria) transcriptome from India | |
Prajapati, Malyaj Rajeev; Singh, Jitender; Kumar, Pankaj; Baranwal, Virendra Kumar | |
通讯作者 | Singh, J |
来源期刊 | ACTA VIROLOGICA
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ISSN | 0001-723X |
EISSN | 1336-2305 |
出版年 | 2022 |
卷号 | 66期号:4页码:376-379 |
英文摘要 | . - Prosopis cineraria Druce (khejri), a member of the Fabaceae family, is an essential legu-minous plant and agri-horticultural crop, which helps to protect the ecology of desert and semi-arid areas. Using the public domain RNA-seq dataset, we aimed to reveal the viral spectrum of khejri, which is still unexplored. Following quality control, 111,497 contigs (> 200 nt) were created by de novo assembly of 24,081,663 reads. The presence of one contig of 10316 nt related to Papaya ringspot virus (PRSV) with sequence identity 83.20%-93.21% was identified through BLASTx analysis, and 3,650 (0.02%) reads were mapped using full-length PRSV sequence as reference. Pairwise sequencing analysis revealed that the nucleotide and amino acid sequences of the complete genome sequence (BK061298) shared 84.30%-95.54% and 86.38%-96.48% similarity with other PRSV genomes, respectively. Phylogenetic analysis revealed that this virus isolate is closely related to PRSV isolate WB (host: Carica papaya; Acc. No. LC482263). Our findings show that Prosopis cineraria (khejri) is a novel host plant of PRSV in India by utilizing khejri transcriptome data and bioinformatics analysis. |
英文关键词 | Prosopis cineraria Potyvirus Papaya ringspot virus RNA-seq phylogenetics India |
类型 | Article |
语种 | 英语 |
收录类别 | SCI-E |
WOS记录号 | WOS:000962398600009 |
WOS类目 | Virology |
WOS研究方向 | Virology |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/391542 |
推荐引用方式 GB/T 7714 | Prajapati, Malyaj Rajeev,Singh, Jitender,Kumar, Pankaj,et al. Genome sequence of a Papaya ringspot virus from khejri (Prosopis cineraria) transcriptome from India[J],2022,66(4):376-379. |
APA | Prajapati, Malyaj Rajeev,Singh, Jitender,Kumar, Pankaj,&Baranwal, Virendra Kumar.(2022).Genome sequence of a Papaya ringspot virus from khejri (Prosopis cineraria) transcriptome from India.ACTA VIROLOGICA,66(4),376-379. |
MLA | Prajapati, Malyaj Rajeev,et al."Genome sequence of a Papaya ringspot virus from khejri (Prosopis cineraria) transcriptome from India".ACTA VIROLOGICA 66.4(2022):376-379. |
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