干旱区生态环境知识资源中心(Arid): Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions

Arid

DOI	10.22456/1679-9216.124079
	Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions
	Soares Brito Rocha, Laysa Mayara; Alvares de Faria, Pedro Jorge; Soares, Rafael Rodrigues; Araujo Junior, Joao Pessoa; Malossi, Camila Dantas; Ullmann, Leila Sabrina; Cristiny Rodrigues Silva, Maria Luana; dos Santos Higino, Severino Silvano; de Azevedo, Sergio Santos; Alves, Clebert Jose
通讯作者	Alves, CJ
来源期刊	ACTA SCIENTIAE VETERINARIAE
ISSN	1678-0345
EISSN	1679-9216
出版年	2022
卷号	50
英文摘要	Background: Leptospirosis is an important infectious disease in goat farming, with a worldwide distribution. It is usually transmitted by rodents and the genital route, may cause reproductive losses, negatively impacting goat fanning. The diagnosis lies on serological, molecular and isolation techniques. Considering the importance of this disease for small ruminants, this work aimed to evaluate the serological, molecular findings and isolation of pathogenic leptospires in the urinary tract (kidney and bladder tissues) of goats. Materials, Methods & Results: Thirty-four adult goats were used for slaughter. Renal samples (n = 34), bladder (n = 34), were collected for isolation of the agent and molecular detection of Leptospira sp. and blood samples (n = 34) for serological testing. The polymerase chain reaction (PCR) was used as a molecular test and the microscopic serum agglutination test (MAT) was used as a serological test. Samples with DNA amplification were subjected to genetic sequencing. The presence of Leptospira DNA was found in the tissues of 8 (23.4%) goats, and of these, only 2 were positive in PCR and MAL There was a slight agreement between the PCR and MAT techniques (k = 0.150; P = 0.436). In 6 (17.6%) samples of renal tissue and 2 (5.8%) bladder samples, Leptospira DNA was detected. The genes in a kidney tissue sample were sequenced and demonstrated 99% similarity to Leptospira interrogans. Anti-Leptospira sp. were detected in 6 (17.6%) of the animals tested. Discussion: Serology identified 3 predominant serogroups: Icterohaemorrhagiae, Tarassovi and Autumnalis, serogroups that are related to the presence of rodents that coexist in rural environments. Autumnalis has been reported in small ruminants, raising the hypothesis that goats are adapted, becoming chronic carriers and possible maintenance hosts. The frequency obtained (17.6%) may be the result of the mixed breed pattern and rustic characteristics inherent to the goat species. Given the characteristics of the semi-arid region, such as low rainfall and high solar incidence, it is essential to use an adapted methodology, with a lower cut-off point (1:50), as the serological titer is an established relationship between the animal species, the level of exposure throughout its evolution and the region studied. Molecular findings and bacterial isolation reveal the agent's ability to colonize the urinary tract of goats. These data show the importance that urine has in the epidemiological chain, being able to transmit the agent through direct contact with this product or through contamination of soil and water. There was no statistical agreement between the diagnostic techniques used in this study, in this case, an association between PCR and MAT is recommended to obtain data with high sensitivity and specificity. A bladder sample was sequenced and showed 99% similarity to Leptospira interrogans. In the semiarid region, the most common form of leptospirosis spread is through the sale of animals in business fairs for breeding, rearing or slaughter, as well as sharing the same property with several breeders. The introduction of chronic and asymptomatic carriers on the properties represents a serious risk for the spread of the disease. The results show the presence of Leptospira spp. in semi-arid goat herds, having as risk factors the presence of rodents and intercropping. The association of MAT and PCR is necessary for a better diagnosis of the disease.
英文关键词	leptospirosis leptospires zoonosis serology molecular detection epidemiology semiarid
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000804934900001
WOS关键词	RISK-FACTORS ; EPIDEMIOLOGIC CHARACTERIZATION ; DAIRY GOATS ; SHEEP ; SEROPREVALENCE ; FREQUENCY ; DIAGNOSIS ; RUMINANTS ; PARAIBA ; STATE
WOS类目	Veterinary Sciences
WOS研究方向	Veterinary Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/391535
推荐引用方式 GB/T 7714	Soares Brito Rocha, Laysa Mayara,Alvares de Faria, Pedro Jorge,Soares, Rafael Rodrigues,et al. Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions[J],2022,50.
APA	Soares Brito Rocha, Laysa Mayara.,Alvares de Faria, Pedro Jorge.,Soares, Rafael Rodrigues.,Araujo Junior, Joao Pessoa.,Malossi, Camila Dantas.,...&Alves, Clebert Jose.(2022).Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions.ACTA SCIENTIAE VETERINARIAE,50.
MLA	Soares Brito Rocha, Laysa Mayara,et al."Leptospira spp. of the Urinary Tract of Female Carrier Goats in Semi-Arid Conditions".ACTA SCIENTIAE VETERINARIAE 50(2022).