干旱区生态环境知识资源中心(Arid): 来自新疆的昆虫病原线虫的鉴定及生物学特性的研究

Arid

	来自新疆的昆虫病原线虫的鉴定及生物学特性的研究
其他题名	Identification and Biological Characteristics of Entomopathogenic Nematode Isolated from Xinjiang
	刘红兵; 刘奇志
来源期刊	中国生物防治学报
ISSN	2095-039X
出版年	2016
卷号	32 期号:1 页码:106-111
中文摘要	通过ITS序列测定,确定了从新疆和田地区间作绿豆的4年枣0~40 cm根际土壤中诱集出的线虫为新疆和田新记录种嗜菌异小杆线虫,命名为新疆枣绿1409品系Heterorhabditis bacteriophora XJZL1409。为确定其生防潜能,本研究以大蜡螟Galleria mellonella L. 4龄末幼虫为寄主,测定其生物学特性。结果表明,该线虫具有较高的侵染力,除剂量10 IJs/larva的校正死亡率为93.33%外,其他剂量浓度的校正死亡率在48 h均已达到100%;该线虫具有较高的耐高温能力,36 ℃下6 h死亡率仅4.93%,致死高温区36~37 ℃;该线虫具有较好的耐干燥能力,在25 ℃、50.5% RH的环境中干燥9 h后,存活率98.82%,复苏后对大蜡螟的致死率仍达到93.33%;该线虫产量3.51*10~5 IJs/g larva。研究表明,该线虫具有生防潜能,作为生物防治制剂更适用于高温干旱地区。
英文摘要	One nematode species was isolated from the rhizosphere soil (040 cm) of four-year jujube trees intercropped with mungbean in Khotan, Xinjiang, China. It was identified as Heterorhabditis bacteriophora and was named as XJZL1409 strain by ITS sequences. It is a new record of nematode Heterorhabditis in the region. Biological characteristics studies showed that the strain was of higher infectivity against Galleria mellonella presented by 100% corrected mortality (CM) in all dosages except the dosage of 10 IJs/larva made 93.33% CM at 48 h. Higher temperature tolerance of this nematode was proved by only 4.93% mortality rate at 36 ℃ for 6 h. The high temperature lethal of XJZL1409 was more than 36 to 37 ℃. It was tolerant to desiccation. The survival rate was still 98.82% after 9 h of desiccation at 50.5% RH in 25 ℃ and the infectivity to G. mellonella still kept to 93.33% at 24 h after recovery from the desiccation. The nematode yield was 3.51*10~5 IJs per gram larva. It was concluded that the nematode had a good potential as a bio-control agent especially suitable to high temperature and dry climate areas.
中文关键词	新疆 ; 嗜菌异小杆线虫 ; 侵染力 ; 耐热性 ; 耐干燥 ; 产量
英文关键词	Xinjiang Heterorhabditis bacteriophora infection heat resistance desiccation tolerance yield
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:5637019
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/378892
作者单位	刘红兵, 中国农业大学农学与生物技术学院, 北京 100193, 中国.; 刘奇志, 中国农业大学农学与生物技术学院, 北京 100193, 中国.
推荐引用方式 GB/T 7714	刘红兵,刘奇志. 来自新疆的昆虫病原线虫的鉴定及生物学特性的研究[J],2016,32(1):106-111.
APA	刘红兵,&刘奇志.(2016).来自新疆的昆虫病原线虫的鉴定及生物学特性的研究.中国生物防治学报,32(1),106-111.
MLA	刘红兵,et al."来自新疆的昆虫病原线虫的鉴定及生物学特性的研究".中国生物防治学报 32.1(2016):106-111.