干旱区生态环境知识资源中心(Arid): 黄土中3株优势细菌的鉴定以及持水效果的初步研究

Arid

	黄土中3株优势细菌的鉴定以及持水效果的初步研究
其他题名	Identification of Three Strains of Predominate Bacteria from Loess and Their Water-Retention Ability in Loess Soil
	王进军; 连宾; 陈骏
来源期刊	农业环境科学学报
ISSN	1672-2043
出版年	2007
卷号	26 期号:4 页码:1575-1578
中文摘要	对黄土中3株优势细菌(DZ023、DZ029、DZ033)进行分子鉴定和16SrDNA同源性分析,同时在实验室条件下模拟测试了这3株富含多糖细菌的持水能力.利用上海申能博彩生物公司试剂盒(K713)进行细菌基因组DNA提取,通过16SrDNA扩增与序列测定,BLAST网上比对表明,3菌株都属于芽孢杆菌属(Bacillus).以甘肃庆阳市黄土样品为试验材料,利用对比法研究了3个菌株在黄土中的持水作用,初步研究结果表明,3个菌株均有明显持水效果,其中DZ029菌株的持水效果最佳.对于研究干旱地区的地质微生物及其应用具有借鉴意义.
英文摘要	Three strains of bacteria(DZ023, DZ029 and DZ033)were isolated and identified from loess soil obtained from Qingyang, Gansu province, north-west part of China. Water-retaining capability is one of the most important indexes that indicate drought resistance capacity of bacteria, so the experimental test for water retention ability in soil using these bacteria were conducted in laboratory. 16SrRNA gene is widely used to classify and identify bacteria because of its special sequence in evolution. By reagent kit K713, three strains of bacteria genome were extracted, amplified and sequenced. The results showed that three strains of bacteria all belonged to Bacillus. The three strains had significant effects on the water conservation compared with the CK, especially the strain DZ029. The mechanism for the ability of bacterial water-retention in soil was attributed to their extracellular polysaccharides which have powerful water-absorbing capacity and also water retention. These can be as a good reference for loess geo-microbe studying area and fighting with drought especially in the dry land area.
中文关键词	黄土 ; 细菌 ; 鉴定 ; 多糖 ; 持水
英文关键词	loess bacteria identification extracellular polysaccharides water-retention
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:2846621
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/378553
作者单位	王进军, 南京师范大学生命科学学院微生物工程重点实验室, 南京, 江苏 210097, 中国.; 连宾, 南京师范大学生命科学学院微生物工程重点实验室, 南京, 江苏 210097, 中国.; 陈骏, 南京大学地球科学系, 南京, 江苏 210093, 中国.
推荐引用方式 GB/T 7714	王进军,连宾,陈骏. 黄土中3株优势细菌的鉴定以及持水效果的初步研究[J],2007,26(4):1575-1578.
APA	王进军,连宾,&陈骏.(2007).黄土中3株优势细菌的鉴定以及持水效果的初步研究.农业环境科学学报,26(4),1575-1578.
MLA	王进军,et al."黄土中3株优势细菌的鉴定以及持水效果的初步研究".农业环境科学学报 26.4(2007):1575-1578.