干旱区生态环境知识资源中心(Arid): 典型喀斯特区云南松林土壤养分和细菌群落对海拔的响应

Arid

	典型喀斯特区云南松林土壤养分和细菌群落对海拔的响应
其他题名	Responses of soil nutrients and microbial community to altitude in typical Pinus yunnanensis forest at rocky desertification region
	隋夕然; 王妍; 刘云根; 张雅洁; 吴丽芳
来源期刊	浙江农业学报
ISSN	1004-1524
出版年	2021
卷号	33 期号:12 页码:2348-2357
中文摘要	以中国西南典型喀斯特区云南松林地为研究对象,采用方差分析、冗余分析和高通量测序等方法,探究土壤养分与细菌群落组成和多样性对不同海拔的响应,为该地区石漠化治理工作和生态系统植被恢复提供参考依据。结果显示:随海拔升高,土壤全碳、全氮、全磷含量呈现先增大后减小的趋势;土壤全钾含量在海拔1 900 m处最高,在海拔1 600 m处最低;土壤速效氮和速效磷含量在海拔1 600 m处最高,分别在海拔1 300 m和海拔1 900 m处最低;速效钾含量在海拔1 900 m处最高,在海拔1 600 m处最低。Chao1指数、Shannon指数随海拔升高先增大后减小,而Simpson指标随海拔升高先减小后增大。在门分类水平上,优势菌门为变形菌门(Proteus)、酸杆菌门(Acidobacteria)、放线菌门(Actinomycetes)和绿弯菌门(Curvularia),分别占比33.37%、24.40%、19.82%、12.06%。其中,变形菌门和酸杆菌门的相对丰度总体随海拔升高先增多后减少,而绿弯菌门的总体变化趋势与之相反。放线菌门在海拔1 600 m的相对丰度最大,其余海拔的相对丰度无显著差异。在纲分类水平上,优势类群包括alpha-变形菌纲(Alphaproteobacteria)、酸杆菌纲(Acidobacteriia)、gamma-变形菌纲(Gammaproteobacteria)、delta-变形菌纲(Deltaproteobacteria),分别占比18.46%、12.72%、7.88%、5.13%。其中,alpha-变形菌纲、gamma-变形菌纲的相对丰度总体随海拔升高先增大后减小,酸杆菌纲的总体变化趋势与此相反,delta-变形菌纲在不同海拔梯度下无明显变化趋势。土壤pH值,及全碳、全氮、全磷、速效氮和速效磷含量与细菌多样性显著(P<0.05)相关,是细菌群落组成变化的主要驱动因子。
英文摘要	In the present study,typical Pinus yunnanensis forest at rocky desertification region in southwest China was selected as the study area,analysis of variance (ANOVA),redundancy analysis (RDA) and high-throughput sequencing methods were applied to explore the response of soil nutrients and bacterial community composition and diversity to different altitudes,which would be helpful for the rocky desertification control and ecosystem vegetation restoration in this area.It was shown that the contents of soil total carbon,total nitrogen and total phosphorus increased fist and decreased then with increasing altitude.The highest content of soil total potassium was at altitude of 1 900 m,yet the lowest was at 1 600 m.The highest content of soil available nitrogen and available phosphorus was both at altitude of 1 600 m,while the lowest was at 1 300 m and 1 900 m,respectively.The highest content of available potassium was at altitude of 1 900 m,yet the lowest was at 1 600 m.The indexes of Chao1,Shannon increased first and then decreased with the elevated altitude,while the Simpson index showed the opposite trend.The dominant phyla of microbial community were Proteus,Acidobacteria,Actinomycetes and Curvularia,which accounted for 33.37%,24.40%,19.82%,12.06%,respectively.The relative abundance of Proteus and Acidobacteria increased first and then decreased with the elevated altitude,while Curvularia showed the opposite trend.The highest relative abundance of Actinomycetes was found at altitude of 1 600 m,yet there was no significant difference within other altitudes.At class level,the dominant microbial community were Alphaproteobacteria,Acidobacteria,Gammaproteobacteria,Deltaproteobacteria,which accounted for 18.46%,12.72%,7.88%,5.13%,respectively.The relative abundance of Alphaproteobacteria and Gammaproteobacteria increased first and then decreased,while Acidobacteria exhibited the opposite.There was no significant difference in the relative abundance of Deltaproteobacteria within different altitudes.Soil pH,and contents of total carbon,total nitrogen,total phosphorus,available nitrogen and available phosphorus were significantly (P<0.05) correlated with the bacterial diversity,and were main driving factors of bacterial community composition.
中文关键词	海拔 ; 土壤养分 ; 高通量测序 ; 细菌
英文关键词	altitude soil nutrients high-throughput sequencing bacteria
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:7111176
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/378044
作者单位	隋夕然, 西南林业大学生态与环境学院, 昆明, 云南 650224, 中国.; 张雅洁, 西南林业大学生态与环境学院, 昆明, 云南 650224, 中国.; 吴丽芳, 西南林业大学生态与环境学院, 昆明, 云南 650224, 中国.; 王妍, 西南林业大学生态与环境学院;;云南省山地农村生态环境演变与污染治理重点实验室, ;;云南省山地农村生态环境演变与污染治理重点实验室, 昆明;;昆明, 云南;;云南 650224;;650224, 中国.; 刘云根, 西南林业大学生态与环境学院;;云南省山地农村生态环境演变与污染治理重点实验室, ;;云南省山地农村生态环境演变与污染治理重点实验室, 昆明;;昆明, 云南;;云南 650224;;650224, 中国.
推荐引用方式 GB/T 7714	隋夕然,王妍,刘云根,等. 典型喀斯特区云南松林土壤养分和细菌群落对海拔的响应[J],2021,33(12):2348-2357.
APA	隋夕然,王妍,刘云根,张雅洁,&吴丽芳.(2021).典型喀斯特区云南松林土壤养分和细菌群落对海拔的响应.浙江农业学报,33(12),2348-2357.
MLA	隋夕然,et al."典型喀斯特区云南松林土壤养分和细菌群落对海拔的响应".浙江农业学报 33.12(2021):2348-2357.