干旱区生态环境知识资源中心(Arid): 黄土高原采煤沉陷对土壤微生物群落的影响

Arid

	黄土高原采煤沉陷对土壤微生物群落的影响
其他题名	Effects of Coal Mining Subsidence on Soil Microbial Community in the Loess Plateau
	马静; 卢永强; 张琦; 李肖肖; 肖栋; 陈浮
来源期刊	土壤学报
ISSN	0564-3929
出版年	2021
卷号	58 期号:5 页码:1278-1288
中文摘要	采矿对环境本底影响极大,近自然恢复是唯一可行的手段。土壤微生物影响物质循环转化,对生态恢复起重要作用。厘清土壤微生物群落如何适应和进化,不同菌群之间如何互作,哪些功能群更适应干旱、贫瘠的环境,对提升矿山生态恢复力至关重要。利用高通量测序和分子生态网络方法探索东坪煤矿和大柳塔煤矿地表沉陷区土壤微生物群落结构与组成、主要种群及与环境因素间的互馈机制。结果表明:沉陷环境对土壤微生物群落多样性、结构和分布模式影响显著,但放线菌门(Actinobacteria)一直最为丰富。东坪煤矿分子生态网络互作关系较大柳塔煤矿更复杂、网络节点和连接数更多。前者的关键菌群属于放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)和奇古菌门(Thaumarchaeota),后者的关键菌群属于变形菌门(Proteobacteria)和放线菌门(Actinobacteria)。此外,网络结构与土壤性状之间存在显著相关性。为适应矿山土壤营养贫瘠,微生物种群间更偏向相互合作,其中Holophagae与Aquabacterium为优势菌属,或可助力矿山生态修复。
英文摘要	【Objective】Mining activity has posed a great impact on the environmental background of the areas. To meet the challenge, the near-natural recovery method might be the only feasible way. Soil microorganisms not only affect the material cycling and transformation of soil substances, but also play an important role in the restoring ecological systems. So to improve resilience of the mining ecological system, it is essential to clarify how soil microbial communities adapt to the environment and evolve therein; how do different microbial groups interacted with each other; and which of their functional groups are more suitable for the arid and barren environments? Moreover, knowledge about interactions between soil microbial communities and their responses to coal mining subsidence are crucial to ecological restoration and resilience of the ecosystems in the semiarid and/or damaged mining areas. However, so far little has been done in this aspect. 【Method】 To explore the structures and compositions of soil microbial communities, and mechanisms of reciprocal feedback between the dominant microbial groups and the environment in the subsidence areas of the Dongping (DP) and Daliuta (DLT) Coal Mines, the methods of high-throughput sequencing and molecular ecological network were adopted. 【Result】 Results showed that the subsidence environment posed a significant impact on the diversity, structure and distribution of the soil microbial communities. Actinobacteria remained to be the dominant phylum in the DP and DLT Coal Mines, whereas DP had two more dominant phyla than DLT did, namely Nitrospira and Cyanobacteria. In the soil of DP, RB41, Solirubrobacter, Roseiflexus, Gaiella and Lysobacter were the genera > 1% in relative abundance, while in the soil of DLT, Nocardioides, RB41, Solirubrobacte and Roseiflexus were. The molecular ecological networks in the soil of the DP Coal Mine interacted more complicatly than those in the soil of the DLT Coal Mine, with more network nodes and connections. The soil microbial groups in the soil of the DP Coal Mine belonged to the phyla of Actinobacteria, Chloroflexi, and Thaumarchaeota, whereas the key species in the soil of the DLT Coal Mine were of the phyla of Proteobacteria and Actinobacteria. Moreover, significant relationships were observed between network structure and soil properties in both mining areas. For instance, among the DP networks, Modules 1, 2 and 3 were significantly and positively related to the soil EC, while module 2 was to the soil water content; Module 6 was significantly and positively related to soil available phosphorus, and Module 1 was to nitrate-nitrogen content; Modules 6 and 7 were significantly and positively related to soil dehydrogenase activity, while Modules 2 and 5 were to activity of soil urease. And among the DLT networks, Module 1 was significantly and positively related to pH and soil temperature, while Module 2 was to soil temperature only; Modules 1 and 2 were significantly and negatively related to soil water content and clay percent, whereas Module 5 was to nitrate-nitrogen content, but reversely. 【Conclusion】In order to adapt to the nutritional infertility of the poor soil in the subsided mining areas, soil microbial communities tended to interact with each other. Holophagae and Aquabacterium were found to be the dominant species, and/or applicable to restoration of the ecological systems in the mining areas in the future.
中文关键词	地表沉陷 ; 群落多样性 ; 分子生态网络 ; 关键菌群 ; 生态恢复
英文关键词	Surface subsidence Community diversity Molecular ecological network Key species Ecological restoration
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Microbiology
CSCD记录号	CSCD:7061262
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377969
作者单位	马静, (徐州)中国矿业大学低碳能源研究院, 徐州, 江苏 221008, 中国.; 陈浮, (徐州)中国矿业大学低碳能源研究院, 徐州, 江苏 221008, 中国.; 卢永强, (徐州)中国矿业大学环境与测绘学院, 徐州, 江苏 221116, 中国.; 张琦, (徐州)中国矿业大学环境与测绘学院, 徐州, 江苏 221116, 中国.; 李肖肖, (徐州)中国矿业大学环境与测绘学院, 徐州, 江苏 221116, 中国.; 肖栋, (徐州)中国矿业大学, 煤炭资源与安全开采国家重点实验室, 徐州, 江苏 221116, 中国.
推荐引用方式 GB/T 7714	马静,卢永强,张琦,等. 黄土高原采煤沉陷对土壤微生物群落的影响[J],2021,58(5):1278-1288.
APA	马静,卢永强,张琦,李肖肖,肖栋,&陈浮.(2021).黄土高原采煤沉陷对土壤微生物群落的影响.土壤学报,58(5),1278-1288.
MLA	马静,et al."黄土高原采煤沉陷对土壤微生物群落的影响".土壤学报 58.5(2021):1278-1288.