干旱区生态环境知识资源中心(Arid): 红砂基因组大小变异及物种分化

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	红砂基因组大小变异及物种分化
其他题名	Genome size variations and species differentiation of Reaumuria soongarica
	范兴科; 燕霞; 冯媛媛; 冉进华; 钱朝菊; 尹晓月; 周姗姗; 房庭舟; 马小飞
来源期刊	生物多样性
ISSN	1005-0094
出版年	2021
卷号	29 期号:10 页码:1308-1320
中文摘要	红砂(Reaumuria soongarica,柽柳科)是广泛分布于亚洲内陆干旱区温带荒漠的建群灌木,该物种内部经历过分化和杂交事件,是研究荒漠植物多样性发生和杂交物种形成的理想模型。然而,红砂不同群体的倍性水平尚不清楚,制约了对其物种形成机制的深入研究。为了确定红砂不同支系的倍性及基因组大小变异模式,本研究以红砂幼苗根尖为实验材料,以野生番茄(Solanum pimpinellifolium)作为内标物种,利用流式细胞术对分别来自北疆支系及其可能的亲本支系(东部和西部支系)的共8个红砂群体进行了DNA 1C-值测定和分析。结果显示:红砂东部支系的DNA 1C-值(1.149 0.012 pg)略小于西部支系的1C-值(1.195 0.031 pg)。北疆支系中阜康(FK)和沙湾(SW)群体的DNA 1C-值处于东、西支系的1C-值之间,而火烧山(HSS)和五彩城(WCC)群体的1C-值接近东、西支系1C-值的2倍。结合之前的分子标记结果,我们推测北疆支系中阜康和沙湾群体为东、西部支系的同倍体杂交种群,而火烧山和五彩城群体属于异源四倍体杂交种群。不同倍性的北疆群体起源于不同的杂交事件,可以划归为不同的物种。
英文摘要	Aims: As a constructive shrub species widely distributed across the temperate deserts of arid Central Asia, Reaumuria soongarica (Tamaricaceae) had undergone divergence and hybridization during its evolutionary history, which make it an ideal model for understanding the molecular basis of biodiversity of desert ecosystems and plant hybrid speciation. However, the ploidy level of different populations of R. soongarica is still unclear, which confines the further study on its speciation mechanism. Methods: To clarify the ploidy level and variation patterns of genome size of different R. soongarica lineages, we investigated the DNA 1C-value of eight R. soongarica populations from the northern lineage (distributed in northern Xinjiang) and its putative parental lineages (the eastern and western lineages) by flow cytometry. The young roots of R. soongarica were selected as the experimental material, and Solanum pimpinellifolium was used as an internal standard species. Results: Our data showed that the DNA 1C-value of the eastern lineage (1.149 0.012 pg) was slightly smaller than that of the western lineage (1.195 0.031 pg), and the DNA 1C-value of the Fukang (FW) and Shawan (SW) populations in the northern lineage was intermediate between that of its parental lineages. However, the DNA 1C-values of the Huoshaoshan (HSS) and Wucaicheng (WCC) populations from the northern lineage were closely twice as those from the eastern and western populations. Conclusion: Considering to the previous results of molecular markers, we infer that the FW and SW populations belong to the homoploid hybrid populations from the eastern and western lineages, while the HSS and WCC populations are allopolyploid hybrid populations. The populations with different ploidy levels in the northern lineage could originate from different hybrid events, which could be classified into different species.
中文关键词	DNA C-值 ; 同倍体杂交物种形成 ; 异源多倍化杂交物种形成 ; 物种多样性
英文关键词	Reaumuria soongarica Reaumuria soongarica DNA C-value homoploid hybrid speciation allopolyploid hybrid speciation species diversity
类型	Article
语种	中文
开放获取类型	Bronze
收录类别	CSCD
WOS类目	Forestry
CSCD记录号	CSCD:7099660
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377887
作者单位	范兴科, 中国科学院西北生态环境资源研究院;;中国科学院大学, 甘肃省寒区旱区逆境生理与生态重点实验室;;, 兰州;;, ;;北京 730000;;100049.; 尹晓月, 中国科学院西北生态环境资源研究院;;中国科学院大学, 甘肃省寒区旱区逆境生理与生态重点实验室;;, 兰州;;, ;;北京 730000;;100049.; 周姗姗, 中国科学院西北生态环境资源研究院;;中国科学院大学, 甘肃省寒区旱区逆境生理与生态重点实验室;;, 兰州;;, ;;北京 730000;;100049.; 房庭舟, 中国科学院西北生态环境资源研究院;;中国科学院大学, 甘肃省寒区旱区逆境生理与生态重点实验室;;, 兰州;;, ;;北京 730000;;100049.; 燕霞, 南通大学生命科学学院, 南通, 江苏 226019, 中国.; 冯媛媛, 中国科学院植物研究所, 系统与进化植物学国家重点实验室, 北京 100093, 中国.; 冉进华, 中国科学院植物研究所, 系统与进化植物学国家重点实验室, 北京 100093, 中国.; 钱朝菊, 中国科学院西北生态环境资源研究院, 甘肃省寒区旱区逆境生理与生态重点实验室, 兰州, 甘肃 730000, 中国.; 马小飞, 中国科学院西北生态环境资源研究院;;南通大学生命科学学院, 甘肃省寒区旱区逆境生理与生态重点实验室;;, 兰州;;南通, ;;江苏 730000;;226019.
推荐引用方式 GB/T 7714	范兴科,燕霞,冯媛媛,等. 红砂基因组大小变异及物种分化[J],2021,29(10):1308-1320.
APA	范兴科.,燕霞.,冯媛媛.,冉进华.,钱朝菊.,...&马小飞.(2021).红砂基因组大小变异及物种分化.生物多样性,29(10),1308-1320.
MLA	范兴科,et al."红砂基因组大小变异及物种分化".生物多样性 29.10(2021):1308-1320.