干旱区生态环境知识资源中心(Arid): 干旱胁迫下葡萄AQP基因家族的鉴定及转录调控网络预测

Arid

	干旱胁迫下葡萄AQP基因家族的鉴定及转录调控网络预测
其他题名	Identification of grapevine AQP family and prediction for transcriptional regulatory network under drought stress
	杨盛迪; 郭大龙; 裴茂松; 刘海楠; 韦同路; 余义和
来源期刊	果树学报
ISSN	1009-9980
出版年	2021
卷号	38 期号:10 页码:1638-1652
中文摘要	【目的】探究干旱胁迫下葡萄AQP的转录调控网络,为后续研究葡萄抗旱基因功能和转录调控机制提供理论依据。【方法】通过生物信息学方法分析葡萄AQP基因家族成员的理化性质、系统进化和染色体定位,进行共线性分析和转录因子调控预测,通过转录组数据分析该基因家族在干旱胁迫和脱落酸(abscisic acid,ABA)处理下的表达情况。【结果】37个VvAQP基因主要分为PIP、TIP、NIP、SIP四类,分别分布在17条染色体上,复制分析结果表明,共有5对节段复制和2对串联复制。蛋白序列分析表明,每个VvAQP蛋白序列中均有1个植物AQP主要内在蛋白(major intrinsic proteins,MIP)的保守结构域特征。通过转录调控网络预测发现,靶向VvAQP基因的转录调控因子主要分为16类。干旱胁迫下,根和叶中大部分VvAQP基因表达持续下调,VvPIP2-3、VvPIP2-6在根中持续上调,7个VvAQP基因受ABA显著诱导。干旱胁迫下15个差异表达的转录因子可能与13个VvAQP基因存在调控关系。【结论】鉴定并提供了葡萄AQP基因家族成员的基本信息,通过转录组数据发现可能参与干旱胁迫的VvAQP基因,并预测了这些基因的转录调控网络。
英文摘要	【Objective】Grape is one of the oldest fruit crops in the world. Most of the grapes in the world are cultivated in arid and semi-arid areas. Facing with the increasingly severe environment on the earth, the grape industry will face a serious impact in the future, drought will become one of the key factors restricting grape production. The aim of this study was to identify members of the AQPs gene family in the whole grape genome. In addition, the transcriptional regulatory network of VvAQPs under drought stress was explored to provide theoretical basis for the subsequent research on the function and the transcriptional regulation mechanism of drought resistance genes in grape.【Methods】Through BLAST Arabidopsis thaliana AQP protein sequences and the HMM file (PF00230), 37 VvAQPs were identified based on the grape genome database. The phylogenetic analysis, gene structure and protein sequence analysis, chromosome location and series replication analysis of grape AQP genes family were performed by using bioinformatics method, interspecific collinear analysis, targeted VvAQPs transcription factor regulatory network analysis. Through drought stress and ABA treatment to further identify the VvAQPs involved in drought, and explore possible transcriptional regulatory relationships. 【Results】 Thirty- seven VvAQPs members were identified from the grape genome. The sequence lengths of VvAQPs varied from 65 to 354, the molecular weights of VvAQPs varied from 6.79 to 37.33 kDa, and the isoelectric points of VvAQPs varied from 4.33 to 9.79. Most of the VvAQPs protein had 6 transmembrane domains. Phylogenetic analysis showed that the family was divided into PIPs, TIPs, NIPs and SIPs. These VvAQPs were distributed among 17 chromosomes, one VvAQP was distributed on 4, 11, 12, 18_random chromosome, and two on 2, 5, 9, 10, 16, 7_random, Un chromosome. There were 3 VvAQPs on 3, 6, 13, 14, and 15, 4VvAQPs on the chr8 chromosomes. VvAQPs were accompanied with 5 pairs of segmental copies and 2 pairs of tandem copies. There were 27 AQP homologous genes between grape and Arabidopsis thaliana, 31 AQP homologous genes in tomato, 36 AQP homologous genes in peach, 55 AQP homologous genes in poplar and 44 AQP homologous genes in kiwifruit, respectively. In addition, the number of homologous genes on the 6, 8 and 13 chromosomes of grape were large, 5 VvAQPs were direct homologous genes with other 6 species, and 7 VvAQPs were homologous with other 5 dicotyledons. Gene structure and protein sequence analysis showed that there was a conserved domain characteristic (MIP) of plant aquaporin in each grape AQP protein sequence and genes from the same subgroup had similar structures. The prediction of transcriptional regulatory network revealed that the transcriptional regulatory factors targeting VvAQPs were mainly divided into 16 types (HD-ZIP, MYB, ERF, bZIP etc.). Combined with transcriptome data, most VvAQPs were significantly down-regulated in roots and leaves under drought stress, and VvAQPs in most roots or leaves were not differentially expressed during the T1 period of drought stress. In roots, VvPIP3-1, VvNIP3-1, VvTIP1-2 and VvTIP2-1 were significantly down-regulated, while VvPIP2-3 showed the opposite trend and was significantly up-regulated. VvNIP2-3, VvPIP1-3, VvNIP1-1 and VvTIP4-1 were significantly down-regulated in the later stage of drought stress, and VvTIP2-2 was only significantly down-regulated in the M4 drought-tolerant genotype. In leaves, VvTIP1-4, VvTIP2-1 and VvPIP3-2 were significantly down-regulated, VvTIP1- 2 and VvNIP3- 1 were up- regulated first and then down- regulated, and most genes of grape PIP subfamily members were significantly down- regulated in later stages, such as VvPIP1- 3, VvPIP1-5, VvPIP2-1, VvPIP2-2, VvPIP2-4and VvPIP2-5. VvAQPs were induced by ABA in different tissues.
中文关键词	葡萄 ; 干旱胁迫 ; 转录因子 ; 调控
英文关键词	AQP Grapevine AQP Drought stress Transcription factor Regulation
类型	Article
语种	中文
收录类别	CSCD
WOS类目	Agriculture
CSCD记录号	CSCD:7074829
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377729
作者单位	杨盛迪, 河南科技大学园艺与植物保护学院;;河南省园艺植物品质调控工程技术研究中心, ;;河南省园艺植物品质调控工程技术研究中心, 洛阳;;洛阳, 河南;;河南 471023;;471023, 中国.; 郭大龙, 河南科技大学园艺与植物保护学院;;河南省园艺植物品质调控工程技术研究中心, ;;河南省园艺植物品质调控工程技术研究中心, 洛阳;;洛阳, 河南;;河南 471023;;471023, 中国.; 裴茂松, 河南科技大学园艺与植物保护学院;;河南省园艺植物品质调控工程技术研究中心, ;;河南省园艺植物品质调控工程技术研究中心, 洛阳;;洛阳, 河南;;河南 471023;;471023, 中国.; 刘海楠, 河南科技大学园艺与植物保护学院;;河南省园艺植物品质调控工程技术研究中心, ;;河南省园艺植物品质调控工程技术研究中心, 洛阳;;洛阳, 河南;;河南 471023;;471023, 中国.; 韦同路, 河南科技大学园艺与植物保护学院;;河南省园艺植物品质调控工程技术研究中心, ;;河南省园艺植物品质调控工程技术研究中心, 洛阳;;洛阳, 河南;;河南 471023;;471023, 中国.; 余义和, 河南科技大学园艺与植物保护学院;;河南省园艺植物品质调控工程技术研究中心, ;;河南省园艺植物品质调控工程技术研究中心, 洛阳;;洛阳, 河南;;河南 471023;;471023, 中国.
推荐引用方式 GB/T 7714	杨盛迪,郭大龙,裴茂松,等. 干旱胁迫下葡萄AQP基因家族的鉴定及转录调控网络预测[J],2021,38(10):1638-1652.
APA	杨盛迪,郭大龙,裴茂松,刘海楠,韦同路,&余义和.(2021).干旱胁迫下葡萄AQP基因家族的鉴定及转录调控网络预测.果树学报,38(10),1638-1652.
MLA	杨盛迪,et al."干旱胁迫下葡萄AQP基因家族的鉴定及转录调控网络预测".果树学报 38.10(2021):1638-1652.