干旱区生态环境知识资源中心(Arid): Phyto-mediated synthesis of CuO nanoparticles using aqueous leaf extract of Artemisia deserti and their anticancer effects on A2780-CP cisplatin-resistant ovarian cancer cells

Arid

DOI	10.1007/s13399-022-02436-x
	Phyto-mediated synthesis of CuO nanoparticles using aqueous leaf extract of Artemisia deserti and their anticancer effects on A2780-CP cisplatin-resistant ovarian cancer cells
	Shahriary, Sepideh; Tafvizi, Farzaneh; Khodarahmi, Parvin; Shaabanzadeh, Masoud
通讯作者	Tafvizi, F (corresponding author)，Islamic Azad Univ, Dept Biol, Parand Branch, Parand, Iran.
来源期刊	BIOMASS CONVERSION AND BIOREFINERY
ISSN	2190-6815
EISSN	2190-6823
出版年	2022-02
英文摘要	Cancer is the second leading cause of death in the word. The failure of the most common therapeutic strategies including chemotherapy and its side effects on normal tissues plus the development of chemoresistance cases has justified the global attempt to find an alternative medicinal approach for cancer treatment. The purpose of this study was to analyze the effect of green-synthesized CuO nanoparticles (CuO NPs) using aqueous leaf extract of the plant Artemisia deserti on A2780-CP cisplatin-resistant ovarian cancer cells. GC-MS analysis of A. deserti extract showed the presence of three main reductive phytocomponents. The properties of synthesized CuO NPs have been characterized using different techniques such as UV-vis, FESEM, TEM, EDX, FTIR, XRD, and DLS. The cytotoxicity effect of CuO NPs has been evaluated by MTT assay. The induction of apoptosis and the cell cycle arrest has been analyzed by flow cytometry and qRT-PCR, respectively. The overall results obtained from MTT assay, Annexin/PI staining, qRT-PCR, cell cycle analysis, and measurement of generated cellular ROS in A2780-CP cells showed that the synthesized CuO NPs could induce apoptosis in A2780-CP cells in a significant level. The results also indicated that the biosynthesized CuO NPs cause negligible amount of cell cytotoxicity on normal healthy human foreskin fibroblasts cells (HFF). These two advantages can make the biogenic synthesized CuO NPs a good candidate for further studies on cancer treatment approaches.
英文关键词	Apoptosis Artemisia deserti CuO nanoparticles Cisplatin resistant Green synthesis Ovarian cancer
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000754946100003
WOS关键词	COPPER-OXIDE NANOPARTICLES ; ANTIBACTERIAL ACTIVITY ; SILVER NANOPARTICLES ; APOPTOSIS INDUCTION ; GREEN SYNTHESIS ; CYTOTOXICITY ; MEDICINE ; PLANT ; SENSITIZATION ; BIOSYNTHESIS
WOS类目	Energy & Fuels ; Engineering, Chemical
WOS研究方向	Energy & Fuels ; Engineering
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377289
作者单位	[Shahriary, Sepideh; Tafvizi, Farzaneh; Khodarahmi, Parvin] Islamic Azad Univ, Dept Biol, Parand Branch, Parand, Iran; [Shaabanzadeh, Masoud] Islamic Azad Univ, Dept Chem, Damghan Branch, Damghan, Iran
推荐引用方式 GB/T 7714	Shahriary, Sepideh,Tafvizi, Farzaneh,Khodarahmi, Parvin,et al. Phyto-mediated synthesis of CuO nanoparticles using aqueous leaf extract of Artemisia deserti and their anticancer effects on A2780-CP cisplatin-resistant ovarian cancer cells[J],2022.
APA	Shahriary, Sepideh,Tafvizi, Farzaneh,Khodarahmi, Parvin,&Shaabanzadeh, Masoud.(2022).Phyto-mediated synthesis of CuO nanoparticles using aqueous leaf extract of Artemisia deserti and their anticancer effects on A2780-CP cisplatin-resistant ovarian cancer cells.BIOMASS CONVERSION AND BIOREFINERY.
MLA	Shahriary, Sepideh,et al."Phyto-mediated synthesis of CuO nanoparticles using aqueous leaf extract of Artemisia deserti and their anticancer effects on A2780-CP cisplatin-resistant ovarian cancer cells".BIOMASS CONVERSION AND BIOREFINERY (2022).