干旱区生态环境知识资源中心(Arid): Expansion of bilin-based red light sensors in the subaerial desert cyanobacterium Nostoc flagelliforme

Arid

DOI	10.1111/1462-2920.15932
	Expansion of bilin-based red light sensors in the subaerial desert cyanobacterium Nostoc flagelliforme
	Xu, Hai-Feng; Dai, Guo-Zheng; Wang, Yu-Jie; Cheng, Chao; Shang, Jin-Long; Li, Ren-Han; Liu, Ke; Duanmu, Deqiang; Qiu, Bao-Sheng
通讯作者	Dai, GZ ; Qiu, BS (corresponding author)，Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China. ; Dai, GZ ; Qiu, BS (corresponding author)，Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
来源期刊	ENVIRONMENTAL MICROBIOLOGY
ISSN	1462-2912
EISSN	1462-2920
出版年	2022-02
英文摘要	Light is the crucial environmental signal for desiccation-tolerant cyanobacteria to activate photosynthesis and prepare for desiccation at dawn. However, the photobiological characteristics of desert cyanobacteria adaptation to one of the harshest habitats on Earth remain unresolved. In this study, we surveyed the genome of a subaerial desert cyanobacterium Nostoc flagelliforme and identified two phytochromes and seven cyanobacteriochromes (CBCRs) with one or more bilin-binding GAF (cGMP phosphodiesterase/adenylyl cyclase/FhlA) domains. Biochemical and spectroscopic analyses of 69 purified GAF-containing proteins from recombinant phycocyanobilin (PCB), biliverdin or phycoerythrobilin-producing Escherichia coli indicated that nine of these proteins bind chromophores. Further investigation revealed that 11 GAFs form covalent adducts responsive to near-UV and visible light: eight GAFs contained PCB chromophores, three GAFs contained biliverdin chromophores and one contained the PCB isomer, phycoviolobilin. Interestingly, COO91_03972 is the first-ever reported GAF-only CBCR capable of sensing five wavelengths of light. Bioinformatics and biochemical analyses revealed that residue P132 of COO91_03972 is essential for chromophore binding to dual-cysteine CBCRs. Furthermore, the complement of N. flagelliforme CBCRs is enriched in red light sensors. We hypothesize that these sensors are critical for the acclimatization of N. flagelliforme to weak light environments at dawn.
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000755953100001
WOS关键词	UV-B ; SIGNAL-TRANSDUCTION ; ADENYLYL-CYCLASE ; BIOSYNTHESIS ; DESICCATION ; PHOTOCONVERSION ; PHYTOCHROMES ; ACCLIMATION ; PERCEPTION ; PHOTOTAXIS
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377278
作者单位	[Xu, Hai-Feng; Dai, Guo-Zheng; Wang, Yu-Jie; Cheng, Chao; Shang, Jin-Long; Li, Ren-Han; Liu, Ke; Qiu, Bao-Sheng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China; [Xu, Hai-Feng; Dai, Guo-Zheng; Wang, Yu-Jie; Cheng, Chao; Shang, Jin-Long; Li, Ren-Han; Liu, Ke; Qiu, Bao-Sheng] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China; [Duanmu, Deqiang] Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Xu, Hai-Feng,Dai, Guo-Zheng,Wang, Yu-Jie,et al. Expansion of bilin-based red light sensors in the subaerial desert cyanobacterium Nostoc flagelliforme[J],2022.
APA	Xu, Hai-Feng.,Dai, Guo-Zheng.,Wang, Yu-Jie.,Cheng, Chao.,Shang, Jin-Long.,...&Qiu, Bao-Sheng.(2022).Expansion of bilin-based red light sensors in the subaerial desert cyanobacterium Nostoc flagelliforme.ENVIRONMENTAL MICROBIOLOGY.
MLA	Xu, Hai-Feng,et al."Expansion of bilin-based red light sensors in the subaerial desert cyanobacterium Nostoc flagelliforme".ENVIRONMENTAL MICROBIOLOGY (2022).