干旱区生态环境知识资源中心(Arid): Downregulation of NADPH-cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides

Arid

DOI	10.1111/1744-7917.12982
	Downregulation of NADPH-cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides
	Qiao, Jian-Wen; Fan, Yong-Liang; Wu, Bing-Jin; Bai, Tian-Tian; Wang, Ying-Hao; Zhang, Zhan-Feng; Wang, Dun; Liu, Tong-Xian
通讯作者	Wang, D (corresponding author)，Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Minist Agr, Yangling 712100, Shaanxi, Peoples R China. ; Wang, D (corresponding author)，Northwest A&F Univ, Key Lab Integrated Pest Management Crops Northwes, Minist Agr, Yangling 712100, Shaanxi, Peoples R China. ; Liu, TX (corresponding author)，Qingdao Agr Univ, Coll Plant Hlth & Med, Key Lab Integrated Crop Pest Management Shandong, Qingdao 266109, Shandong, Peoples R China.
来源期刊	INSECT SCIENCE
ISSN	1672-9609
EISSN	1744-7917
出版年	2022-01
英文摘要	Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is involved in the metabolism of endogenous and exogenous substances, and detoxification of insecticides. RNA interference (RNAi) of CPR in certain insects causes developmental defects and enhanced susceptibility to insecticides. However, the CPR of Acyrthosiphon pisum has not been characterized, and its function is still not understood. In this study, we investigated the biochemical functions of A. pisum CPR (ApCPR). ApCPR was found to be transcribed in all developmental stages and was abundant in the embryo stage, and in the gut, head, and abdominal cuticle. After optimizing the dose and silencing duration of RNAi for downregulating ApCPR, we found that ApCPR suppression resulted in a significant decrease in the production of cuticular and internal hydrocarbon contents, and of cuticular waxy coatings. Deficiency in cuticular hydrocarbons (CHCs) decreased the survival rate of A. pisum under desiccation stress and increased its susceptibility to contact insecticides. Moreover, desiccation stress induced a significant increase in ApCPR mRNA levels. We further confirmed that ApCPR participates in CHC production. These results indicate that ApCPR modulates CHC production, desiccation tolerance, and insecticide susceptibility in A. pisum, and presents a novel target for pest control.
英文关键词	Acyrthosiphon pisum desiccation tolerance hydrocarbon insecticide susceptibility NADPH-cytochrome P450 reductase
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000741481400001
WOS关键词	DOUBLE-STRANDED-RNA ; PEA APHID ; HYDROCARBON PRODUCTION ; NUCLEASE ACTIVITY ; CHEMICAL ECOLOGY ; NEXT-GENERATION ; DESERT LOCUST ; GENE ; KNOCKDOWN ; IDENTIFICATION
WOS类目	Entomology
WOS研究方向	Entomology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377150
作者单位	[Qiao, Jian-Wen; Fan, Yong-Liang; Wu, Bing-Jin; Bai, Tian-Tian; Wang, Ying-Hao; Zhang, Zhan-Feng; Wang, Dun] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Minist Agr, Yangling 712100, Shaanxi, Peoples R China; [Qiao, Jian-Wen; Fan, Yong-Liang; Wu, Bing-Jin; Bai, Tian-Tian; Wang, Ying-Hao; Zhang, Zhan-Feng; Wang, Dun] Northwest A&F Univ, Key Lab Integrated Pest Management Crops Northwes, Minist Agr, Yangling 712100, Shaanxi, Peoples R China; [Liu, Tong-Xian] Qingdao Agr Univ, Coll Plant Hlth & Med, Key Lab Integrated Crop Pest Management Shandong, Qingdao 266109, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Jian-Wen,Fan, Yong-Liang,Wu, Bing-Jin,et al. Downregulation of NADPH-cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides[J],2022.
APA	Qiao, Jian-Wen.,Fan, Yong-Liang.,Wu, Bing-Jin.,Bai, Tian-Tian.,Wang, Ying-Hao.,...&Liu, Tong-Xian.(2022).Downregulation of NADPH-cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides.INSECT SCIENCE.
MLA	Qiao, Jian-Wen,et al."Downregulation of NADPH-cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides".INSECT SCIENCE (2022).