干旱区生态环境知识资源中心(Arid): Invitro culture in combination with aeroponics is an efficient means of mass propagation of Sarcostemma acidum: a rare medicinal plant of Indian arid zone

Arid

DOI	10.1007/s11627-021-10245-6
	Invitro culture in combination with aeroponics is an efficient means of mass propagation of Sarcostemma acidum: a rare medicinal plant of Indian arid zone
	Choudhary, Preeti; Kataria, Vinod
通讯作者	Kataria, V
来源期刊	IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN	1054-5476
EISSN	1475-2689
出版年	2022
英文摘要	Invitro culture in combination with aeroponics is observed to be an efficient means for mass propagation of Sarcostemma acidum in the present investigation. S. acidum is a rare leafless xerophytic shrub of the family Apocynaceae, commonly known as Som-lata or khir-kheep, a source of medicines and the religious drink Somaras. Aeroponic technique was used for the induction of adventitious roots from stem cuttings of field-grown plants for the development of quality plants in the greenhouse as a source of explants for micropropagation. Among various concentrations of IBA, NAA and NOA used for induction of adventitious rooting from stem cuttings 3.0 g L-1 NAA were found to be most suitable with average 90% induction response and 14 adventitious roots. Surface-sterilized nodal shoot segments of 6 to 7 cm length were inoculated on basic and modified Murashige and Skoog's medium containing 3% (w/v) sucrose, 0.8% (w/v) agar, and various concentrations of BAP and kinetin for activation of axillary shoot buds. Shoots were mass multiplied on a modified MS (MMS) medium containing 3.5 mg L-1 BAP and 0.01 mg L-1 IAA. Invitro multiplied shoots were rooted invitro on half-strength MS medium containing 0.25 mg L-1 NAA. Ninety percent of invitro regenerated plantlets were hardened successfully on Soilrite in the greenhouse and survived on garden and field soils. This protocol will be useful for invitro propagation of S. acidum for mass plantations in the desert ecosystem for prospects.
英文关键词	Adventitious roots Aeroponics Propagation Plant growth regulators Invitro rooting Acclimatization
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000742307500001
WOS关键词	LEPTADENIA-RETICULATA ; SHOOT REGENERATION ; GROWTH-REGULATORS ; NODAL EXPLANTS ; MULTIPLICATION ; AUXIN ; MICROPROPAGATION ; MICROCUTTINGS
WOS类目	Plant Sciences ; Cell Biology ; Developmental Biology
WOS研究方向	Plant Sciences ; Cell Biology ; Developmental Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377134
推荐引用方式 GB/T 7714	Choudhary, Preeti,Kataria, Vinod. Invitro culture in combination with aeroponics is an efficient means of mass propagation of Sarcostemma acidum: a rare medicinal plant of Indian arid zone[J],2022.
APA	Choudhary, Preeti,&Kataria, Vinod.(2022).Invitro culture in combination with aeroponics is an efficient means of mass propagation of Sarcostemma acidum: a rare medicinal plant of Indian arid zone.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT.
MLA	Choudhary, Preeti,et al."Invitro culture in combination with aeroponics is an efficient means of mass propagation of Sarcostemma acidum: a rare medicinal plant of Indian arid zone".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT (2022).