干旱区生态环境知识资源中心(Arid): Synergetic effects of TDZ with various phytohormones on high-frequency plant regeneration from mature nodal explants of Capparis decidua and their ex vivo implications

Arid

DOI	10.1007/s11240-022-02234-3
	Synergetic effects of TDZ with various phytohormones on high-frequency plant regeneration from mature nodal explants of Capparis decidua and their ex vivo implications
	Ram, Kheta; Patel, Ashok Kumar; Choudhary, Sumitra Kumari; Shekhawat, N. S.
通讯作者	Ram, K (corresponding author)，Jai Narain Vyas Univ, UGC Ctr Adv Study CAS, Dept Bot, Biotechnol Unit, Jodhpur 342005, Rajasthan, India.
来源期刊	PLANT CELL TISSUE AND ORGAN CULTURE
ISSN	0167-6857
EISSN	1573-5044
出版年	2022-01
英文摘要	Key message Capparis decidua is a well-known shrub of arid/semi-arid horticulture and agroforestry. This paper evidences an excellent example of technology-transfer from lab to land for commercial propagation using mature nodal explants and synergism of PGRs followed by ex vitro rooting and concurrent acclimatization. Capparis decidua (Forssk.) Edgew., is a well-known ornamental shrub of arid and semi-arid horticulture and agroforestry. This paper represents the synergism of thidiazuron (TDZ) with auxins and cytokinins for the development of an efficient shoot regeneration system from mature nodal explants. The optimum axillary shoot-bud differentiation occurred on Murashige and Skoog (Physiol Plant 15:473-497, 1962) medium augmented with 0.48 mg L-1 TDZ and 0.1 mg L-1 alpha-naphthaleneacetic acid (NAA). Shoot multiplication through repetitive transfer of mother explants was optimally achieved on MS medium having 0.24 mg L-1 TDZ, 0.25 mg L-1 6-benzyladenine (BA), 0.1 mg L-1 NAA, on which the maximum number of shoots (15.4 +/- 0.92) were obtained after the third passage. Shoot amplification through subculturing was achieved on MS medium with 1.2 mu g L-1 TDZ, 0.75 mg L-1 BA, 0.2 mg L-1 Kinetin (Kin) and 150 mg L-1 of ammonium sulphate [(NH4)(2)SO4], on which the maximum shoot multiplication rate (40.6 +/- 1.2 per culture vessel) was obtained. Microshoots were successfully rooted by in vitro and ex vitro methods. During in vitro rooting, more than 80% of the microshoots were rooted on MS1/4 strength medium supplemented with 5.0 mg L-1 Indole-3-butyric acid (IBA) and 200 mg L-1 of activated charcoal (AC). For ex vitro rooting, pulse-treatment of 500 mg L-1 IBA and 100 mg L-1 beta-naphthoxyacetic acid (NOA) for 5 min was found to be the best on which 94.66 +/- 4.16% of the shoots produced 5.3 +/- 0.64 roots per shoot of an average length 4.75 +/- 0.89 cm. In comparison to plantlets developed from in vitro rooting, percentage survival of plants that rooted ex vitro was significantly (P < 0.05) higher. More than 93% of the micropropagated plants successfully survived in the field conditions. The young Ker fruits can be harvested twice a year (Mar-Apr and Jul-Aug). The present market prices of young and processed/dried Ker fruits are INR 250 to 300 and INR 1450 to 1500 per kg, respectively. The present protocol can be used for large-scale commercial propagation of this important ornamental and horticultural shrub.
英文关键词	Capparis decidua EVRCA Ornamental shrub Shoot multiplication Thidiazuron
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000746326700003
WOS关键词	IN-VITRO PROPAGATION ; FORSK EDGEW ; MICROPROPAGATION ; TREE ; THIDIAZURON ; CULTURE ; SHRUB
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377089
作者单位	[Ram, Kheta; Patel, Ashok Kumar; Choudhary, Sumitra Kumari; Shekhawat, N. S.] Jai Narain Vyas Univ, UGC Ctr Adv Study CAS, Dept Bot, Biotechnol Unit, Jodhpur 342005, Rajasthan, India
推荐引用方式 GB/T 7714	Ram, Kheta,Patel, Ashok Kumar,Choudhary, Sumitra Kumari,et al. Synergetic effects of TDZ with various phytohormones on high-frequency plant regeneration from mature nodal explants of Capparis decidua and their ex vivo implications[J],2022.
APA	Ram, Kheta,Patel, Ashok Kumar,Choudhary, Sumitra Kumari,&Shekhawat, N. S..(2022).Synergetic effects of TDZ with various phytohormones on high-frequency plant regeneration from mature nodal explants of Capparis decidua and their ex vivo implications.PLANT CELL TISSUE AND ORGAN CULTURE.
MLA	Ram, Kheta,et al."Synergetic effects of TDZ with various phytohormones on high-frequency plant regeneration from mature nodal explants of Capparis decidua and their ex vivo implications".PLANT CELL TISSUE AND ORGAN CULTURE (2022).