干旱区生态环境知识资源中心(Arid): Diversity and bioprospecting for industrial hydrolytic enzymes of microbial communities isolated from deserted areas of south-east Morocco

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DOI	10.3934/microbiol.2022002
	Diversity and bioprospecting for industrial hydrolytic enzymes of microbial communities isolated from deserted areas of south-east Morocco
	Manni, Amina; Filali-Maltouf, Abdelkarim
通讯作者	Filali-Maltouf, A (corresponding author)，Mohammed V Univ, Dept Biol, Lab Microbiol & Mol Biol, Av Ibn Batouta BP 1014, Rabat, Morocco.
来源期刊	AIMS MICROBIOLOGY
ISSN	2471-1888
出版年	2022
卷号	8 期号:1
英文摘要	The current study aimed to analyze bacterial communities' diversity and abundance in three different deserted areas (Merzouga, Mhamid Elghizlane, and Erg lihoud) located in Moroccan Sahara, as well as to investigate osmotolerant microorganisms producing hydrolytic enzymes. The isolates were taxonomically affiliated using 16S rRNA gene sequencing. Four different hydrolase activities (amylase, lipase, cellulase, and protease) and osmotic stress tolerance were evaluated. The phylogenetic analysis of 364 screened isolates belonged to three phyla (Firmicutes 73%, Proteobacteria 26% and Actinobacteria 1%) and 18 different genera, from Bacillus, Ornithinibacillus, Paenibacillus, Geobacillus, Pseudomonas, Acinetobacter, Agrobacterium, Arthrobacter, Paenarthrobacter, Enterobacter, Staphylococcus, Envinia, Herbasprillum, Ocuria, Massilia, Planomicrobium, Hodococcus, and Stenotrophomonas. The results detected a high proportion of osmotolerant and enzymes producing bacteria, many isolates can tolerate up to 55 degrees C (40%, 28%, and 30% in Merzouga, Mhamid Elghizlane, and Erg lihoudi, respectively). Meanwhile, the salinity tolerance reached 12% in some isolates with different proportions in each site, 29% in Merzouga, 24% in Mhamid Elghizlane, and 9% in Erg lihoudi. Furthermore, the enzymatic tests showed the presence of an amylolytic, lipolytic, cellulolytic, proteolytic activities in 20%, 31%, 63% and 72% of total strains, respectively. As a result, the present study is thus a preliminary yet critical step towards identifying the best bacterial candidates for further biotechnological applications.
英文关键词	bacterial diversity desert enzyme activity stress tolerance Sahara
类型	Article
语种	英语
开放获取类型	gold
收录类别	ESCI
WOS记录号	WOS:000747201400001
WOS关键词	THERMOPHILIC BACTERIA ; SOIL ; MICROORGANISMS ; TOLERANT ; REGIONS ; AMYLASE ; SAMPLES ; SAHARA ; LIPASE ; LAKE
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/377011
作者单位	[Manni, Amina; Filali-Maltouf, Abdelkarim] Mohammed V Univ, Dept Biol, Lab Microbiol & Mol Biol, Av Ibn Batouta BP 1014, Rabat, Morocco
推荐引用方式 GB/T 7714	Manni, Amina,Filali-Maltouf, Abdelkarim. Diversity and bioprospecting for industrial hydrolytic enzymes of microbial communities isolated from deserted areas of south-east Morocco[J],2022,8(1).
APA	Manni, Amina,&Filali-Maltouf, Abdelkarim.(2022).Diversity and bioprospecting for industrial hydrolytic enzymes of microbial communities isolated from deserted areas of south-east Morocco.AIMS MICROBIOLOGY,8(1).
MLA	Manni, Amina,et al."Diversity and bioprospecting for industrial hydrolytic enzymes of microbial communities isolated from deserted areas of south-east Morocco".AIMS MICROBIOLOGY 8.1(2022).