干旱区生态环境知识资源中心(Arid): Structural and Functional Characterization of Camelus dromedarius Glutathione Transferase M1-1

Arid

DOI	10.3390/life12010106
	Structural and Functional Characterization of Camelus dromedarius Glutathione Transferase M1-1
	Perperopoulou, Fereniki; Poudel, Nirmal; Papageorgiou, Anastassios C.; Ataya, Farid S.; Labrou, Nikolaos E.
通讯作者	Labrou, NE (corresponding author)，Agr Univ Athens, Sch Appl Biol & Biotechnol, Dept Biotechnol, Lab Enzyme Technol, 75 Iera Odos St, GR-11855 Athens, Greece.
来源期刊	LIFE-BASEL
EISSN	2075-1729
出版年	2022
卷号	12 期号:1
英文摘要	Glutathione transferases (GSTs; EC. 2.5.1.18) are a large family of multifunctional enzymes that play crucial roles in the metabolism and inactivation of a broad range of xenobiotic compounds. In the present work, we report the kinetic and structural characterization of the isoenzyme GSTM1-1 from Camelus dromedarius (CdGSTM1-1). The CdGS tau M1-1 was expressed in E. coli BL21 (DE3) and was purified by affinity chromatography. Kinetics analysis showed that the enzyme displays a relative narrow substrate specificity and restricted ability to bind xenobiotic compounds. The crystal structures of CdGS tau M1-1 were determined by X-ray crystallography in complex with the substrate (GSH) or the reaction product (S-p-nitrobenzyl-GSH), providing snapshots of the induced-fit catalytic mechanism. The thermodynamic stability of CdGSTM1-1 was investigated using differential scanning fluorimetry (DSF) in the absence and in presence of GSH and S-p-nitrobenzyl-GSH and revealed that the enzyme's structure is significantly stabilized by its ligands. The results of the present study advance the understanding of camelid GST detoxification mechanisms and their contribution to abiotic stress adaptation in harsh desert conditions.
英文关键词	abiotic stress biotic stress glutathione transferase herbicide detoxification pesticide determination xenobiotics
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000757035100001
WOS关键词	S-TRANSFERASE ; MECHANISM ; REVEALS ; P1-1 ; SITE
WOS类目	Biology ; Microbiology
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376696
作者单位	[Perperopoulou, Fereniki; Labrou, Nikolaos E.] Agr Univ Athens, Sch Appl Biol & Biotechnol, Dept Biotechnol, Lab Enzyme Technol, 75 Iera Odos St, GR-11855 Athens, Greece; [Poudel, Nirmal; Papageorgiou, Anastassios C.] Univ Turku, Turku Biosci Ctr, Turku 20521, Finland; [Poudel, Nirmal; Papageorgiou, Anastassios C.] Abo Akad Univ, Turku 20521, Finland; [Ataya, Farid S.] King Saud Univ, Coll Sci, Biochem Dept, POB 2455, Riyadh 11451, Saudi Arabia
推荐引用方式 GB/T 7714	Perperopoulou, Fereniki,Poudel, Nirmal,Papageorgiou, Anastassios C.,et al. Structural and Functional Characterization of Camelus dromedarius Glutathione Transferase M1-1[J],2022,12(1).
APA	Perperopoulou, Fereniki,Poudel, Nirmal,Papageorgiou, Anastassios C.,Ataya, Farid S.,&Labrou, Nikolaos E..(2022).Structural and Functional Characterization of Camelus dromedarius Glutathione Transferase M1-1.LIFE-BASEL,12(1).
MLA	Perperopoulou, Fereniki,et al."Structural and Functional Characterization of Camelus dromedarius Glutathione Transferase M1-1".LIFE-BASEL 12.1(2022).