干旱区生态环境知识资源中心(Arid): Methylation Pattern Mediated by m(6)A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma

Arid

DOI	10.1155/2022/2930310
	Methylation Pattern Mediated by m(6)A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma
	Jiang, Feng; Hu, Yifang; Liu, Xiaoqin; Wang, Ming; Wu, Chuyan
通讯作者	Wu, CY (corresponding author)，Nanjing Med Univ, Affiliated Hosp 1, Dept Rehabil Med, Nanjing 210029, Peoples R China.
来源期刊	OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
ISSN	1942-0900
EISSN	1942-0994
出版年	2022
卷号	2022
英文摘要	Background. Recent research has established the existence of epigenetic modulation of the immune response. The possible involvement of RNA-n6-methyladenosine (m(6)A) alteration in tumor microenvironment (TME) cell invasion, on the other hand, is unknown. Methods. Based on 23 m(6)A regulators, we examined the alteration patterns of m(6)A in 629 LUAD tissues and comprehensively connected these modification patterns with TME cell invasion characteristics. The m(6)A score was calculated, and the m(6)A modification pattern of a single tumor was quantified using principal component analysis. Then, we further verified the expression of m(6)A related enzymes and the role hub gene (NOL10) closely related to survival in lung cancer cell lines. Results. Three separate m(6)A alteration modes have been discovered. TME cell invasion characteristics in the three modes were very similar to the three immunological phenotypes of tumors: immunological rejection, immunological inflammation, and immunological desert. We show that assessing the m(6)A modification pattern in a single tumor may help predict tumor inflammatory stage, subtype, TME interstitial activity, and prognosis. TME phenotypic inflammation is indicated by a high m(6)A score, which is characterized by elevated mutation load and immunological activation. The low m(6)A subtype showed matrix activation and ineffective immune infiltration, indicating that the TME phenotype of noninflammation and immunological rejection had a poor survival probability. Increased neoantigen burden was also linked to a high m(6)A score. Patients with a higher m(6)A score saw substantial therapeutic and clinical improvements. And reducing hub gene NOL10 expression substantially inhibited lung cancer cell growth and migration. Conclusions. This research shows that m(6)A alteration is critical in the creation of TME variety and complexity. The analysis of a single tumor's m(6)A alteration pattern will aid in improving our knowledge of TME invasion features and guiding more effective immunotherapy tactics.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000746476500002
WOS关键词	IMMUNE CONTEXTURE ; CANCER ; CELLS ; IMPACT ; SET
WOS类目	Cell Biology
WOS研究方向	Cell Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376612
作者单位	[Jiang, Feng] Fudan Univ, Obstet & Gynecol Hosp, Dept Neonatol, Shanghai 200011, Peoples R China; [Hu, Yifang] Nanjing Med Univ, Affiliated Hosp 1, Dept Geriatr, Nanjing 210029, Peoples R China; [Liu, Xiaoqin] Nanjing Med Univ, Clin Med Coll, Affiliated Nanjing Drum Tower Hosp, Dept Pulm & Crit Care Med, Nanjing 210008, Jiangsu, Peoples R China; [Wang, Ming] Nanjing Med Univ, Affiliated Hosp 1, Plast Surg Dept, Nanjing 210029, Peoples R China; [Wu, Chuyan] Nanjing Med Univ, Affiliated Hosp 1, Dept Rehabil Med, Nanjing 210029, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Feng,Hu, Yifang,Liu, Xiaoqin,et al. Methylation Pattern Mediated by m(6)A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma[J],2022,2022.
APA	Jiang, Feng,Hu, Yifang,Liu, Xiaoqin,Wang, Ming,&Wu, Chuyan.(2022).Methylation Pattern Mediated by m(6)A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma.OXIDATIVE MEDICINE AND CELLULAR LONGEVITY,2022.
MLA	Jiang, Feng,et al."Methylation Pattern Mediated by m(6)A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma".OXIDATIVE MEDICINE AND CELLULAR LONGEVITY 2022(2022).