干旱区生态环境知识资源中心(Arid): Sea Anemones Responding to Sex Hormones, Oxybenzone, and Benzyl Butyl Phthalate: Transcriptional Profiling and in Silico Modelling Provide Clues to Decipher Endocrine Disruption in Cnidarians

Arid

DOI	10.3389/fgene.2021.793306
	Sea Anemones Responding to Sex Hormones, Oxybenzone, and Benzyl Butyl Phthalate: Transcriptional Profiling and in Silico Modelling Provide Clues to Decipher Endocrine Disruption in Cnidarians
	Morgan, Michael B.; Ross, James; Ellwanger, Joseph; Phrommala, Rebecca Martin; Youngblood, Hannah; Qualley, Dominic; Williams, Jacob
通讯作者	Morgan, MB (corresponding author)，Berry Coll, Dept Biol, Mt Berry, GA 30149 USA. ; Morgan, MB (corresponding author)，Berry Coll, Dept Chem & Biochem, Mt Berry, GA 30149 USA.
来源期刊	FRONTIERS IN GENETICS
EISSN	1664-8021
出版年	2022
卷号	12
英文摘要	Endocrine disruption is suspected in cnidarians, but questions remain how occurs. Steroid sex hormones are detected in corals and sea anemones even though these animals do not have estrogen receptors and their repertoire of steroidogenic enzymes appears to be incomplete. Pathways associated with sex hormone biosynthesis and sterol signaling are an understudied area in cnidarian biology. The objective of this study was to identify a suite of genes that can be linked to exposure of endocrine disruptors. Exaiptasia diaphana were exposed to nominal 20ppb concentrations of estradiol (E2), testosterone (T), cholesterol, oxybenzone (BP-3), or benzyl butyl phthalate (BBP) for 4 h. Eleven genes of interest (GOIs) were chosen from a previously generated EST library. The GOIs are 17 beta-hydroxysteroid dehydrogenases type 14 (17 beta HSD14) and type 12 (17 beta HSD12), Niemann-Pick C type 2 (NPC2), Equistatin (EI), Complement component C3 (C3), Cathepsin L (CTSL), Patched domain-containing protein 3 (PTCH3), Smoothened (SMO), Desert Hedgehog (DHH), Zinc finger protein GLI2 (GLI2), and Vitellogenin (VTG). These GOIs were selected because of functional associations with steroid hormone biosynthesis; cholesterol binding/transport; immunity; phagocytosis; or Hedgehog signaling. Quantitative Real-Time PCR quantified expression of GOIs. In silico modelling utilized protein structures from Protein Data Bank as well as creating protein structures with SWISS-MODEL. Results show transcription of steroidogenic enzymes, and cholesterol binding/transport proteins have similar transcription profiles for E2, T, and cholesterol treatments, but different profiles when BP-3 or BBP is present. C3 expression can differentiate between exposures to BP-3 versus BBP as well as exposure to cholesterol versus sex hormones. In silico modelling revealed all ligands (E2, T, cholesterol, BBP, and BP-3) have favorable binding affinities with 17 beta HSD14, 17 beta HSD12, NPC2, SMO, and PTCH proteins. VTG expression was down-regulated in the sterol treatments but up-regulated in BP-3 and BBP treatments. In summary, these eleven GOIs collectively generate unique transcriptional profiles capable of discriminating between the five chemical exposures used in this investigation. This suite of GOIs are candidate biomarkers for detecting transcriptional changes in steroidogenesis, gametogenesis, sterol transport, and Hedgehog signaling. Detection of disruptions in these pathways offers new insight into endocrine disruption in cnidarians.
英文关键词	endocrine disruption chemicals cnidaria in silico modelling and docking biomarkers sex hormones xenobiotics transcriptional profiling hedgehog signaling
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000757346600001
WOS关键词	REPRESENTATIONAL DIFFERENCE ANALYSIS ; EUPHYLLIA-ANCORA CNIDARIA ; GREAT-BARRIER-REEF ; SCLERACTINIAN CORAL ; SEWAGE POLLUTION ; SONIC HEDGEHOG ; RECEPTOR-ALPHA ; ACTINIA-EQUINA ; BASAL METAZOAN ; NATIONAL-PARK
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376571
作者单位	[Morgan, Michael B.; Ross, James; Ellwanger, Joseph; Phrommala, Rebecca Martin; Youngblood, Hannah; Williams, Jacob] Berry Coll, Dept Biol, Mt Berry, GA 30149 USA; [Morgan, Michael B.; Ross, James; Youngblood, Hannah; Qualley, Dominic] Berry Coll, Dept Chem & Biochem, Mt Berry, GA 30149 USA; [Ross, James] Emory Univ, Sch Med, Emory Vaccine Ctr, Dept Microbiol & Immunol, Atlanta, GA 30322 USA; [Youngblood, Hannah] Augusta Univ, Dept Cellular Biol & Anat, Augusta, GA USA
推荐引用方式 GB/T 7714	Morgan, Michael B.,Ross, James,Ellwanger, Joseph,et al. Sea Anemones Responding to Sex Hormones, Oxybenzone, and Benzyl Butyl Phthalate: Transcriptional Profiling and in Silico Modelling Provide Clues to Decipher Endocrine Disruption in Cnidarians[J],2022,12.
APA	Morgan, Michael B..,Ross, James.,Ellwanger, Joseph.,Phrommala, Rebecca Martin.,Youngblood, Hannah.,...&Williams, Jacob.(2022).Sea Anemones Responding to Sex Hormones, Oxybenzone, and Benzyl Butyl Phthalate: Transcriptional Profiling and in Silico Modelling Provide Clues to Decipher Endocrine Disruption in Cnidarians.FRONTIERS IN GENETICS,12.
MLA	Morgan, Michael B.,et al."Sea Anemones Responding to Sex Hormones, Oxybenzone, and Benzyl Butyl Phthalate: Transcriptional Profiling and in Silico Modelling Provide Clues to Decipher Endocrine Disruption in Cnidarians".FRONTIERS IN GENETICS 12(2022).