干旱区生态环境知识资源中心(Arid): Characterization of the genome and serine protease of a novel Bacillus subtilis isolate

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DOI	10.1007/s10482-021-01696-3
	Characterization of the genome and serine protease of a novel Bacillus subtilis isolate
	Khan, Zahoor; Shafique, Maryam; Saleem, Faizan; Azim, M. Kamran; Jabeen, Nusrat; Naz, Sehar Afshan
通讯作者	Shafique, M (corresponding author)，Fed Urdu Univ Arts Sci & Technol, Dept Microbiol, Karachi 75300, Pakistan.
来源期刊	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN	0003-6072
EISSN	1572-9699
出版年	2022
卷号	115 期号:2 页码:281-295
英文摘要	Current study was undertaken to carry out the genome-wide analysis of a multipotent isolate from desert soil which was previously identified as Bacillus tequilensis based on 16S rDNA analysis. This study also aims to characterize the serine protease and its biocatalytic potentials implying a combination of empirical and in-silico approaches. Next generation sequencing and short read de novo assembly generated the 4,235,084 bp draft genome of Bacillus sp. ZMS-2. Genome sequence analysis by digital DNA:DNA hybridization (dDDH) and average nucleotide identity classified the isolate as Bacillus subtilis ZMS-2 (Bioproject ID: PRJNA691551). Genome annotation revealed 10 antibiotic resistance genes, 8 antibiotic/antifungal gene clusters and 25 genes encoding proteases including subtilisin E, an extracellular alkaline protease. This extracellular protease (ZMS-2 protease) was produced using a statistically optimized medium, purified partially and characterized as alkaline serine protease. The partially purified ZMS-2 protease (780 U/mL) showed a 21 mm zone of casein hydrolysis and dehaired goat skin by pulling out hair with roots. These catalytic potentials of ZMS-2 protease were further confirmed using scanning electron microscopy of casein beads and dehaired skin. The study concludes B. subtilis ZMS-2 as a potent producer of a protease with promising potentials of commercial importance.
英文关键词	Bacillus subtilis ZMS-2 Genome-wide ZMS-2 protease Caseinolytic Keratinolytic
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000742571800003
WOS关键词	ALKALINE PROTEASE ; PURIFICATION ; PREDICTION ; ROLES
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376556
作者单位	[Khan, Zahoor; Jabeen, Nusrat] Univ Karachi, Dept Microbiol, Karachi 75270, Pakistan; [Khan, Zahoor; Shafique, Maryam; Naz, Sehar Afshan] Fed Urdu Univ Arts Sci & Technol, Dept Microbiol, Karachi 75300, Pakistan; [Saleem, Faizan; Azim, M. Kamran] Mohammad Ali Jinnah Univ, Dept Biosci, Karachi 75400, Pakistan; [Saleem, Faizan] McMaster Univ, Dept Biol, Hamilton, ON L8S 4L8, Canada
推荐引用方式 GB/T 7714	Khan, Zahoor,Shafique, Maryam,Saleem, Faizan,et al. Characterization of the genome and serine protease of a novel Bacillus subtilis isolate[J],2022,115(2):281-295.
APA	Khan, Zahoor,Shafique, Maryam,Saleem, Faizan,Azim, M. Kamran,Jabeen, Nusrat,&Naz, Sehar Afshan.(2022).Characterization of the genome and serine protease of a novel Bacillus subtilis isolate.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,115(2),281-295.
MLA	Khan, Zahoor,et al."Characterization of the genome and serine protease of a novel Bacillus subtilis isolate".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 115.2(2022):281-295.