干旱区生态环境知识资源中心(Arid): Cloning and Functional Characterization of a Double-Stranded RNA-Degrading Nuclease in the Tawny Crazy Ant (Nylanderia fulva)

Arid

DOI	10.3389/fphys.2022.833652
	Cloning and Functional Characterization of a Double-Stranded RNA-Degrading Nuclease in the Tawny Crazy Ant (Nylanderia fulva)
	Lei, Jiaxin; Tan, Yongan; List, Fabian; Puckett, Robert; Tarone, Aaron M.; Vargo, Edward L.; Zhu-Salzman, Keyan
通讯作者	Zhu-Salzman, K (corresponding author)，Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA.
来源期刊	FRONTIERS IN PHYSIOLOGY
EISSN	1664-042X
出版年	2022
卷号	13
英文摘要	RNA interference is a powerful tool that post-transcriptionally silences target genes. However, silencing efficacy varies greatly among different insect species. Recently, we attempted to knock down some housekeeping genes in the tawny crazy ant (Nylanderia fulva), a relatively new invasive species in the southern United States, but only achieved relatively low silencing efficiency when dsRNA was orally administered. Here, we detected divalent cation-dependent, dsRNA-degrading activity in the midgut fluid of worker ants in ex vivo assays. To determine whether dsRNA degradation could contribute to low effectiveness of oral RNAi in N. fulva, we cloned its sole dsRNase gene (NfdsRNase). The deduced amino acid sequence contained a signal peptide and an endonuclease domain. Sequence alignment indicated a high degree of similarity with well-characterized dsRNases, particularly the six key residues at active sites. We also identified dsRNase homologs from five other ant species and found a tight phylogenetic relationship among ant dsRNases. NfdsRNase is expressed predominantly in the abdomen of worker ants. Oral delivery of dsRNA of NfdsRNase significantly reduced the expression of NfdsRNase transcripts, and substantially suppressed dsRNA-degrading activity of worker ants' midgut fluids as well. Our data suggest that dsRNA stability in the alimentary tract is an important factor for gene silencing efficiency in N. fulva, and that blocking NfdsRNase in gut lumen could potentially improve RNAi, a novel pest management tactic in control of N. fulva and other ant species.
英文关键词	Nylanderia fulva RNAi efficiency dsRNA stability dsRNase midgut fluid
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000760602600001
WOS关键词	DESERT LOCUST ; INSECT PEST ; INTERFERENCE ; IDENTIFICATION ; EFFICIENCY ; EXPRESSION ; ALIGNMENT ; GUT
WOS类目	Physiology
WOS研究方向	Physiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376499
作者单位	[Lei, Jiaxin; Tan, Yongan; List, Fabian; Puckett, Robert; Tarone, Aaron M.; Vargo, Edward L.; Zhu-Salzman, Keyan] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA; [Tan, Yongan] Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Peoples R China
推荐引用方式 GB/T 7714	Lei, Jiaxin,Tan, Yongan,List, Fabian,et al. Cloning and Functional Characterization of a Double-Stranded RNA-Degrading Nuclease in the Tawny Crazy Ant (Nylanderia fulva)[J],2022,13.
APA	Lei, Jiaxin.,Tan, Yongan.,List, Fabian.,Puckett, Robert.,Tarone, Aaron M..,...&Zhu-Salzman, Keyan.(2022).Cloning and Functional Characterization of a Double-Stranded RNA-Degrading Nuclease in the Tawny Crazy Ant (Nylanderia fulva).FRONTIERS IN PHYSIOLOGY,13.
MLA	Lei, Jiaxin,et al."Cloning and Functional Characterization of a Double-Stranded RNA-Degrading Nuclease in the Tawny Crazy Ant (Nylanderia fulva)".FRONTIERS IN PHYSIOLOGY 13(2022).