干旱区生态环境知识资源中心(Arid): Comoclathrin, a novel potent skin-whitening agent produced by endophytic Comoclathris strains associated with Andalusia desert plants

Arid

DOI	10.1038/s41598-022-05448-9
	Comoclathrin, a novel potent skin-whitening agent produced by endophytic Comoclathris strains associated with Andalusia desert plants
	Georgousaki, Katerina; Gonzalez-Menendez, Victor; Tormo, Jose R.; Tsafantakis, Nikolaos; Mackenzie, Thomas A.; Martin, Jesus; Gumeni, Sentiljana; Trougakos, Ioannis P.; Reyes, Fernando; Fokialakis, Nikolas; Genilloud, Olga
通讯作者	Fokialakis, N (corresponding author)，Natl & Kapodistrian Univ Athens, Dept Pharm, Div Pharmacognosy & Nat Prod Chem, Athens, Greece. ; Genilloud, O (corresponding author)，Fdn MEDINA, Hlth Sci Technol Pk, Granada, Spain.
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2022
卷号	12 期号:1
英文摘要	As part of our screening program for the discovery of molecules of microbial origin with skin-whitening activity, 142 diverse fungal endophytes from a wide variety of Andalusia arid plants were screened, applying the OSMAC approach. The fungal strains CF-090361 and CF-090766, isolated from xerophytic plants, were selected as the most promising, while phylogenetic analysis revealed that both strains could represent a new species within the genus Comoclathris. The effect of different fermentation conditions on the production of tyrosinase inhibitory activity was examined, in order to identify the optimum cultivation conditions. LCMS based metabolomics was applied to determine significant differences between the strains and fermentation conditions, and to identify potential bioactive secondary metabolites. Bioassay-guided purification of the main active components led to the isolation of three new compounds (1-3), along with the known compounds graphostrin B (4) and brevianamide M (5). Compound 1 (Comoclathrin) demonstrated the strongest anti-tyrosinase activity (IC50 0.16 mu M), which was 90-times higher than kojic acid (IC50 14.07 mu M) used as positive control. Additionally, comoclathrin showed no significant cytotoxicity against a panel of cancer cell lines (HepG2, A2058, A549, MCF-7 and MIA PaCa-2) and normal BJ fibroblasts. These properties render comoclathrin an excellent development candidate as whitening agent.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000749168300005
WOS关键词	ENHANCEMENT
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376490
作者单位	[Georgousaki, Katerina; Tsafantakis, Nikolaos; Fokialakis, Nikolas] Natl & Kapodistrian Univ Athens, Dept Pharm, Div Pharmacognosy & Nat Prod Chem, Athens, Greece; [Georgousaki, Katerina; Gonzalez-Menendez, Victor; Tormo, Jose R.; Mackenzie, Thomas A.; Martin, Jesus; Reyes, Fernando; Genilloud, Olga] Fdn MEDINA, Hlth Sci Technol Pk, Granada, Spain; [Gumeni, Sentiljana; Trougakos, Ioannis P.] Natl & Kapodistrian Univ Athens, Fac Biol, Dept Cell Biol & Biophys, Athens, Greece
推荐引用方式 GB/T 7714	Georgousaki, Katerina,Gonzalez-Menendez, Victor,Tormo, Jose R.,et al. Comoclathrin, a novel potent skin-whitening agent produced by endophytic Comoclathris strains associated with Andalusia desert plants[J],2022,12(1).
APA	Georgousaki, Katerina.,Gonzalez-Menendez, Victor.,Tormo, Jose R..,Tsafantakis, Nikolaos.,Mackenzie, Thomas A..,...&Genilloud, Olga.(2022).Comoclathrin, a novel potent skin-whitening agent produced by endophytic Comoclathris strains associated with Andalusia desert plants.SCIENTIFIC REPORTS,12(1).
MLA	Georgousaki, Katerina,et al."Comoclathrin, a novel potent skin-whitening agent produced by endophytic Comoclathris strains associated with Andalusia desert plants".SCIENTIFIC REPORTS 12.1(2022).