干旱区生态环境知识资源中心(Arid): Roots of the xerophyte Panicum turgidum host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria

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DOI	10.1016/j.sjbs.2021.09.020
	Roots of the xerophyte Panicum turgidum host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria
	Guesmi, Sihem; Najjari, Afef; Pujic, Petar; Ghedira, Kais; Ouertani, Rania; Jabberi, Marwa; Cherif, Ameur; Normand, Philippe; Sghaier, Haitham
通讯作者	Guesmi, S ; Sghaier, H (corresponding author)，Natl Ctr Nucl Sci & Technol CNSTN, Lab Energy & Matter Dev Nucl Sci LR16CNSTN02, Sidi Thabet Technopk, Sidi Thabet 2020, Tunisia.
来源期刊	SAUDI JOURNAL OF BIOLOGICAL SCIENCES
ISSN	1319-562X
EISSN	2213-7106
出版年	2022
卷号	29 期号:2 页码:1260-1268
英文摘要	Bacterial communities associated with roots of Panicum turgidum, exposed to arid conditions, were investigated with a combination of cultural and metataxonomic approaches. Traditional culture-based techniques were used and 32 isolates from the irradiated roots were identified as belonging to Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria phyla. Four actinobacterial strains were shown to be ionizing-radiation (IR)-resistant: Microbacterium sp. PT8 (4.8 kGy (kGy)), Micrococcus sp. PT11 (4.4 kGy), Kocuria rhizophila PT10 (2.9 kGy) and Promicromonospora panici PT9T (2.6 kGy), based on the D10 dose necessary for a 90% reduction in colony forming units (CFU). Concerning the investigation of microbial communities in situ, metataxonomic analyses of the diversity of IR-resistant microorganisms associated with irradiated roots revealed a marked dominance of Actinobacteria (46.6%) and Proteobacteria (31.5%) compared to Bacteroidetes (4.6%) and Firmicutes (3.2%). Gamma irradiation not only changed the structure of bacterial communities, but also affected their functional properties. Comparative analyses of metabolic profiles indicated the induction of several pathways related to adaptation to oxidative stress in irradiated roots, such as DNA repair, secondary metabolites synthesis, reactive oxygen species (ROS)-mitigating enzymes, etc. P. turgidum is emblematic of desert-adapted plants. Until now, there is no other work that has focused on the microbial profile of irradiated roots of this xerophyte. (c) 2021 Published by Elsevier B.V. on behalf of King Saud University.
英文关键词	Culture approach Desiccation Metabolic profiles Metataxonomic Panicum turgidum Radioresistant
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000754638700016
WOS关键词	RIBOSOMAL-RNA SEQUENCES ; METAGENOMIC ANALYSIS ; SP-NOV. ; DESERT ; DIVERSITY ; LIFE ; MICROORGANISMS ; DATABASE ; GROWTH ; TREE
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376422
作者单位	[Guesmi, Sihem] Natl Agron Inst Tunisia INAT, Ave Charles Nicolle, Tunis 1082, Mahrajene, Tunisia; [Guesmi, Sihem; Jabberi, Marwa; Sghaier, Haitham] Natl Ctr Nucl Sci & Technol CNSTN, Lab Energy & Matter Dev Nucl Sci LR16CNSTN02, Sidi Thabet Technopk, Sidi Thabet 2020, Tunisia; [Najjari, Afef] Univ Tunis El Manar, Fac Sci Tunis, LR03ES03 Microorganismes & Biomol Act, Tunis 2092, Tunisia; [Pujic, Petar; Normand, Philippe] Univ Lyon, F-69622 Lyon, France; [Pujic, Petar; Normand, Philippe] Univ Lyon 1, Villeurbanne, France; [Pujic, Petar; Normand, Philippe] CNRS, UMR5557, Ecol Microbienne, Villeurbanne, France; [Pujic, Petar; Normand, Philippe] INRA, UMR1418, Villeurbanne, France; [Ghedira, Kais] Univ Tunis El Manar, Inst Pasteur Tunis, Lab Bioinformat Biomath & Biostat LR16IPT09, Tunis 1002, Tunisia; [Ouertani, Rania; Cherif, Ameur; Sghaier, Haitham] Univ Manouba, ISBST, Biotechpole Sidi Thabet, BVBGR LR11ES31, Ariana 2020, Tunisia; [Jabberi, Marwa] Univ Carthage, Fac Sci Bizerte, Biochem & Mol Biol Lab, Ri...
推荐引用方式 GB/T 7714	Guesmi, Sihem,Najjari, Afef,Pujic, Petar,et al. Roots of the xerophyte Panicum turgidum host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria[J],2022,29(2):1260-1268.
APA	Guesmi, Sihem.,Najjari, Afef.,Pujic, Petar.,Ghedira, Kais.,Ouertani, Rania.,...&Sghaier, Haitham.(2022).Roots of the xerophyte Panicum turgidum host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria.SAUDI JOURNAL OF BIOLOGICAL SCIENCES,29(2),1260-1268.
MLA	Guesmi, Sihem,et al."Roots of the xerophyte Panicum turgidum host a cohort of ionizing-radiation-resistant biotechnologically-valuable bacteria".SAUDI JOURNAL OF BIOLOGICAL SCIENCES 29.2(2022):1260-1268.