干旱区生态环境知识资源中心(Arid): Application of Solid-Phase Extraction and High-Performance Liquid Chromatography with Fluorescence Detection to Analyze Eleven Bisphenols in Amniotic Fluid Samples Collected during Amniocentesis

Arid

DOI	10.3390/ijerph19042309
	Application of Solid-Phase Extraction and High-Performance Liquid Chromatography with Fluorescence Detection to Analyze Eleven Bisphenols in Amniotic Fluid Samples Collected during Amniocentesis
	Tuzimski, Tomasz; Szubartowski, Szymon
通讯作者	Tuzimski, T
来源期刊	INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
EISSN	1660-4601
出版年	2022
卷号	19 期号:4
英文摘要	Amniocentesis involves taking a sample of the amniotic fluid in order to perform a karyotype test and diagnose any genetic defects that may affect the fetus. Amniotic fluid has been collected from patients with an indication for amniocentesis in the 15-26th week of pregnancy. A simple and sensitive high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method for identification and quantification of eleven selected bisphenols in amniotic fluid samples is proposed. The proposed method involved protein precipitation using acetonitrile, and next the extraction and concentration of analytes by solid-phase extraction (SPE). The solid-phase extraction (SPE) procedure with application of Oasis HLB SPE columns performed well for the majority of the analytes, with recoveries in the range of 67-121% and relative standard deviations (RSD%) less than 16%. The limits of detection (LODs) and quantification (LOQs) of all the investigated analytes were in the range of 0.8-2.5 ng mL(-1) and 2.4-7.5 ng mL(-1) (curves constructed in methanol) and 1.1-5.2 ng mL(-1) and 3.2-15.6 ng mL(-1) (curves constructed in the amniotic fluid), respectively. The method was validated at the following two concentration levels: 10 ng mL(-1) (2 x LOQ) and 20 ng mL(-1) (4 LOQ). The results confirm the validity of the SPE procedure and HPLC-FLD method for identification and quantification of bisphenols in amniotic fluid samples collected during an amniocentesis. The result obtained show that HPLC-FLD is a useful method for determination of bisphenol residues at nanogram per milliliter concentrations in amniotic fluid samples. Residues of five analytes (BADGE center dot 2H(2)O, BPAF, BADGE, BADGE center dot H2O center dot HCl and BADGE center dot 2HCl) were detected in amniotic fluid samples. Additionally, the harmfulness of bisphenols as potential pathogens that may cause karyotype disorders and contribute to preterm birth was estimated.
英文关键词	bisphenols amniotic fluid samples fluorescence detector (FLD) solid-phase extraction (SPE) Scherzo SM-C18 amniocentesis
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E ; SSCI
WOS记录号	WOS:000762320200001
WOS关键词	UNITED-STATES ; BREAST-MILK ; URINE ; SERUM
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376380
推荐引用方式 GB/T 7714	Tuzimski, Tomasz,Szubartowski, Szymon. Application of Solid-Phase Extraction and High-Performance Liquid Chromatography with Fluorescence Detection to Analyze Eleven Bisphenols in Amniotic Fluid Samples Collected during Amniocentesis[J],2022,19(4).
APA	Tuzimski, Tomasz,&Szubartowski, Szymon.(2022).Application of Solid-Phase Extraction and High-Performance Liquid Chromatography with Fluorescence Detection to Analyze Eleven Bisphenols in Amniotic Fluid Samples Collected during Amniocentesis.INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH,19(4).
MLA	Tuzimski, Tomasz,et al."Application of Solid-Phase Extraction and High-Performance Liquid Chromatography with Fluorescence Detection to Analyze Eleven Bisphenols in Amniotic Fluid Samples Collected during Amniocentesis".INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 19.4(2022).