干旱区生态环境知识资源中心(Arid): Endophytic Bacteria Colonizing the Petiole of the Desert Plant Zygophyllum dumosum Boiss: Possible Role in Mitigating Stress

Arid

DOI	10.3390/plants11040484
	Endophytic Bacteria Colonizing the Petiole of the Desert Plant Zygophyllum dumosum Boiss: Possible Role in Mitigating Stress
	Srinivasan, Jansirani; Khadka, Janardan; Novoplansky, Nurit; Gillor, Osnat; Grafi, Gideon
通讯作者	Grafi, G (corresponding author)，Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, French Associates Inst Agr & Biotechnol Drylands, IL-84990 Ben Gurion, Israel.
来源期刊	PLANTS-BASEL
EISSN	2223-7747
出版年	2022
卷号	11 期号:4
英文摘要	Zygophyllum dumosum is a dominant shrub in the Negev Desert whose survival is accomplished by multiple mechanisms including abscission of leaflets to reduce whole plant transpiration while leaving the fleshy, wax-covered petioles alive but dormant during the dry season. Petioles that can survive for two full growing seasons maintain cell component integrity and resume metabolic activity at the beginning of the winter. This remarkable survival prompted us to investigate endophytic bacteria colonizing the internal tissues of the petiole and assess their role in stress tolerance. Twenty-one distinct endophytes were isolated by culturing from surface-sterile petioles and identified by sequencing of the 16S rDNA. Sequence alignments and the phylogenetic tree clustered the isolated endophytes into two phyla, Firmicutes and Actinobacteria. Most isolated endophytes displayed a relatively slow growth on nutrient agar, which was accelerated by adding petiole extracts. Metabolic analysis of selected endophytes showed several common metabolites whose level is affected by petiole extract in a species-dependent manner including phosphoric acid, pyroglutamic acid, and glutamic acid. Other metabolites appear to be endophyte-specific metabolites, such as proline and trehalose, which were implicated in stress tolerance. These results demonstrate the existence of multiple endophytic bacteria colonizing Z. dumosum petioles with the potential role in maintaining cell integrity and functionality via synthesis of multiple beneficial metabolites that mitigate stress and contribute to stress tolerance.
英文关键词	desert plants phyllosphere endophytic bacteria Zygophyllum dumosum Actinobacteria Firmicutes 16S rRNA genes endophyte metabolites stress-related metabolites
类型	Article
语种	英语
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000764642200001
WOS关键词	HALOPHYTE SALICORNIA-EUROPAEA ; SP NOV. ; PSYCHROPHILIC BACTERIUM ; EMENDED DESCRIPTION ; NEGEV DESERT ; GEN. NOV. ; SOIL ; RHIZOSPHERE ; METABOLISM ; TREHALOSE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376357
作者单位	[Srinivasan, Jansirani; Khadka, Janardan; Novoplansky, Nurit; Grafi, Gideon] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, French Associates Inst Agr & Biotechnol Drylands, IL-84990 Ben Gurion, Israel; [Srinivasan, Jansirani; Gillor, Osnat] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Zuckerberg Inst Water Res, IL-84990 Ben Gurion, Israel
推荐引用方式 GB/T 7714	Srinivasan, Jansirani,Khadka, Janardan,Novoplansky, Nurit,et al. Endophytic Bacteria Colonizing the Petiole of the Desert Plant Zygophyllum dumosum Boiss: Possible Role in Mitigating Stress[J],2022,11(4).
APA	Srinivasan, Jansirani,Khadka, Janardan,Novoplansky, Nurit,Gillor, Osnat,&Grafi, Gideon.(2022).Endophytic Bacteria Colonizing the Petiole of the Desert Plant Zygophyllum dumosum Boiss: Possible Role in Mitigating Stress.PLANTS-BASEL,11(4).
MLA	Srinivasan, Jansirani,et al."Endophytic Bacteria Colonizing the Petiole of the Desert Plant Zygophyllum dumosum Boiss: Possible Role in Mitigating Stress".PLANTS-BASEL 11.4(2022).