干旱区生态环境知识资源中心(Arid): Genetic Diversity and Population Structure of Doum Palm (Hyphaene compressa) Using Genotyping by Sequencing

Arid

DOI	10.3389/fgene.2022.762202
	Genetic Diversity and Population Structure of Doum Palm (Hyphaene compressa) Using Genotyping by Sequencing
	Omire, Agnes; Neondo, Johnstone; Budambula, Nancy L. M.; Wangai, Laura; Ogada, Stephen; Mweu, Cecilia
通讯作者	Mweu, C
来源期刊	FRONTIERS IN GENETICS
EISSN	1664-8021
出版年	2022
卷号	13
英文摘要	Doum palm (Hyphaene compressa) is a perennial economic plant primarily growing in Kenya's Arid and Semi-Arid Lands (ASALs). It is heavily relied upon for food, animal feed, construction materials and medicine, making it an ideal plant for resource sustainability. However, the limited information on its genetic resources has hindered its breeding and conservation studies. This study used the genotyping by sequencing approach to identify Single Nucleotide Polymorphisms. These SNPs were further used to assess the genetic diversity and population structure of 96 H. compressa accessions from Coastal, Northern and Eastern ASAL regions of Kenya using two approaches; reference-based and de novo-based assemblies. STRUCTURE analysis grouped the sampled accessions into two genetic clusters (Cluster 1 and Cluster 2). Cluster 1 included accessions from the Northern region, whereas Cluster 2 included all accessions from Eastern and Coastal regions. Accessions from Kwale (Coastal) had mixed ancestry from both Cluster 1 and Cluster 2. These STRUCTURE findings were further supported by principal components analysis, discriminant analysis of principal components and phylogenetic analysis. Analysis of molecular variance indicated greater genetic variation within populations (92.7%) than among populations (7.3%). An overall F-ST of 0.074 was observed, signifying moderate genetic differentiation among populations. The results of this study will provide information useful in breeding, marker-assisted selection and conservation management of H. compressa.
英文关键词	genetic diversity GBS single nucleotide polymorphisms population structure Hyphaene compressa doum palm
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000760699200001
WOS关键词	DISCOVERY ; EVOLUTION
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376330
推荐引用方式 GB/T 7714	Omire, Agnes,Neondo, Johnstone,Budambula, Nancy L. M.,et al. Genetic Diversity and Population Structure of Doum Palm (Hyphaene compressa) Using Genotyping by Sequencing[J],2022,13.
APA	Omire, Agnes,Neondo, Johnstone,Budambula, Nancy L. M.,Wangai, Laura,Ogada, Stephen,&Mweu, Cecilia.(2022).Genetic Diversity and Population Structure of Doum Palm (Hyphaene compressa) Using Genotyping by Sequencing.FRONTIERS IN GENETICS,13.
MLA	Omire, Agnes,et al."Genetic Diversity and Population Structure of Doum Palm (Hyphaene compressa) Using Genotyping by Sequencing".FRONTIERS IN GENETICS 13(2022).