干旱区生态环境知识资源中心(Arid): Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Arid

DOI	10.1038/s41598-022-06193-9
	Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
	Shakeel, Muhammad; Du, Juan; Li, Shang-Wei; Zhou, Yuan-Jin; Sarwar, Naeem; Guo, Xiaolan
通讯作者	Li, SW (corresponding author)，Guizhou Univ, Inst Entomol, Prov Key Lab Agr Pest Management Mt Reg, Guiyang 550025, Guizhou, Peoples R China.
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2022
卷号	12 期号:1
英文摘要	Parental RNAi (pRNAi) is a response of RNA interference in which treated insect pests progenies showed a gene silencing phenotypes. pRNAi of CmGNA gene has been studied in Cnaphalocrocis medinalis via injection. Our results showed significant reduction in ovulation per female that was 26% and 35.26% in G1 and G2 generations, respectively. Significant reduction of hatched eggs per female were observed 23.53% and 45.26% as compared to control in G1-G2 generations, respectively. We also observed the significant variation in the sex ratio between female (40% and 53%) in G1-G2 generations, and in male (65%) in G1 generation as compared to control. Our results also demonstrated the significant larval mortality (63% and 55%) and pupal mortality (55% and 41%), and significant reduction of mRNA expression level in G1 and G2 generations. Our findings have confirmed that effectiveness of pRNAi induced silencing on the CmGNA target gene in G1-G2 generations of C. medinalis. These results suggested the potential role of pRNAi in insect pest resistance management strategies.
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000756832600077
WOS关键词	DOUBLE-STRANDED-RNA ; WESTERN CORN-ROOTWORM ; GLUCOSAMINE 6-PHOSPHATE ACETYLASE ; DIABROTICA-VIRGIFERA-VIRGIFERA ; CHITIN SYNTHASE ; GUENEE LEPIDOPTERA ; DESERT LOCUST ; INSECT PESTS ; GAP GENE ; DSRNA
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376315
作者单位	[Shakeel, Muhammad; Du, Juan; Li, Shang-Wei; Zhou, Yuan-Jin] Guizhou Univ, Inst Entomol, Prov Key Lab Agr Pest Management Mt Reg, Guiyang 550025, Guizhou, Peoples R China; [Sarwar, Naeem] Bahauddin Zakariya Univ, Dept Agron, Multan 60800, Pakistan; [Guo, Xiaolan] Guizhou Univ, Coll Forestry, Huaxi 550025, Peoples R China
推荐引用方式 GB/T 7714	Shakeel, Muhammad,Du, Juan,Li, Shang-Wei,et al. Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)[J],2022,12(1).
APA	Shakeel, Muhammad,Du, Juan,Li, Shang-Wei,Zhou, Yuan-Jin,Sarwar, Naeem,&Guo, Xiaolan.(2022).Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).SCIENTIFIC REPORTS,12(1).
MLA	Shakeel, Muhammad,et al."Glucosamine-6-phosphate N-acetyltransferase gene silencing by parental RNA interference in rice leaf folder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)".SCIENTIFIC REPORTS 12.1(2022).