干旱区生态环境知识资源中心(Arid): Transcriptome Analysis of Bread Wheat Genotype KRL3-4 Provides a New Insight Into Regulatory Mechanisms Associated With Sodicity (High pH) Tolerance

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DOI	10.3389/fgene.2021.782366
	Transcriptome Analysis of Bread Wheat Genotype KRL3-4 Provides a New Insight Into Regulatory Mechanisms Associated With Sodicity (High pH) Tolerance
	Prasad, Geeta; Mittal, Shikha; Kumar, Arvind; Chauhan, Divya; Sahu, Tanmaya Kumar; Kumar, Sundeep; Singh, Rakesh; Yadav, Mahesh C.; Singh, Amit Kumar
通讯作者	Singh, AK (corresponding author)，ICAR NBPGR, Div Genom Resources, New Delhi, India. ; Kumar, A (corresponding author)，ICAR Cent Soil Salin Res Inst, Karnal, Haryana, India.
来源期刊	FRONTIERS IN GENETICS
EISSN	1664-8021
出版年	2022
卷号	12
英文摘要	Globally, sodicity is one of the major abiotic stresses limiting the wheat productivity in arid and semi-arid regions. With due consideration, an investigation of the complex gene network associated with sodicity stress tolerance is required to identify transcriptional changes in plants during abiotic stress conditions. For this purpose, we sequenced the flag leaf transcriptome of a highly tolerant bread wheat germplasm (KRL 3-4) in order to extend our knowledge and better understanding of the molecular basis of sodicity tolerance. A total of 1,980 genes were differentially expressed in the flag leaf due to sodicity stress. Among these genes, 872 DEGs were upregulated and 1,108 were downregulated. Furthermore, annotation of DEGs revealed that a total of 1,384 genes were assigned to 2,267 GO terms corresponding to 502 (biological process), 638 (cellular component), and 1,127 (molecular function). GO annotation also revealed the involvement of genes related to several transcription factors; the important ones are expansins, peroxidase, glutathione-S-transferase, and metal ion transporters in response to sodicity. Additionally, from 127 KEGG pathways, only 40 were confidently enriched at a p-value <0.05 covering the five main KEGG categories of metabolism, i.e., environmental information processing, genetic information processing, organismal systems, and cellular processes. Most enriched pathways were prioritized using MapMan software and revealed that lipid metabolism, nutrient uptake, and protein homeostasis were paramount. We have also found 39 SNPs that mapped to the important sodicity stress-responsive genes associated with various pathways such as ROS scavenging, serine/threonine protein kinase, calcium signaling, and metal ion transporters. In a nutshell, only 19 important candidate genes contributing to sodicity tolerance in bread wheat were identified, and these genes might be helpful for better understanding and further improvement of sodicity tolerance in bread wheat.
英文关键词	KRL 3-4 transcriptome sodicity DEGs ROS
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000760730900001
WOS关键词	RESPONSIVE GENE-EXPRESSION ; RICE ORYZA-SATIVA ; SALT STRESS ; TRITICUM-AESTIVUM ; PHOSPHATE TRANSPORTERS ; ARABINOGALACTAN-PROTEINS ; PLANT TOLERANCE ; PLASMA-MEMBRANE ; ARABIDOPSIS ; IDENTIFICATION
WOS类目	Genetics & Heredity
WOS研究方向	Genetics & Heredity
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376309
作者单位	[Prasad, Geeta; Mittal, Shikha; Chauhan, Divya; Sahu, Tanmaya Kumar; Kumar, Sundeep; Singh, Rakesh; Yadav, Mahesh C.; Singh, Amit Kumar] ICAR NBPGR, Div Genom Resources, New Delhi, India; [Kumar, Arvind] ICAR Cent Soil Salin Res Inst, Karnal, Haryana, India
推荐引用方式 GB/T 7714	Prasad, Geeta,Mittal, Shikha,Kumar, Arvind,et al. Transcriptome Analysis of Bread Wheat Genotype KRL3-4 Provides a New Insight Into Regulatory Mechanisms Associated With Sodicity (High pH) Tolerance[J],2022,12.
APA	Prasad, Geeta.,Mittal, Shikha.,Kumar, Arvind.,Chauhan, Divya.,Sahu, Tanmaya Kumar.,...&Singh, Amit Kumar.(2022).Transcriptome Analysis of Bread Wheat Genotype KRL3-4 Provides a New Insight Into Regulatory Mechanisms Associated With Sodicity (High pH) Tolerance.FRONTIERS IN GENETICS,12.
MLA	Prasad, Geeta,et al."Transcriptome Analysis of Bread Wheat Genotype KRL3-4 Provides a New Insight Into Regulatory Mechanisms Associated With Sodicity (High pH) Tolerance".FRONTIERS IN GENETICS 12(2022).