干旱区生态环境知识资源中心(Arid): Isolation of rhizosheath and analysis of microbial community structure around roots of Stipa grandis

Arid

DOI	10.1038/s41598-022-06708-4
	Isolation of rhizosheath and analysis of microbial community structure around roots of Stipa grandis
	Zhu, Ai-Min; Wu, Qian; Liu, Hai-Li; Sun, Hai-Lian; Han, Guo-Dong
通讯作者	Han, GD (corresponding author)，Inner Mongolia Agr Univ, Key Lab Forage Cultivat Proc & Higher Efficient U, Coll Grassland Resources & Environm,Inner Mongoli, Key Lab Grassland Resources,Minist Educ China,Min, Hohhot 010019, Peoples R China.
来源期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
出版年	2022
卷号	12 期号:1
英文摘要	Root zone microbial structure is particularly complex in plants with rhizosheaths, and greater understanding of the rhizosheath may play an important role in the future development of sustainable agricultural practices. However, one important reason to focus study on rhizosheath microbial structure is that there is no definite method for rhizosheath separation. The aim of this study was to explore rhizosheath isolation methods and the diversity characteristics of microorganisms around the rhizosphere. In this study, we isolated the rhizosheath of Stipa grandis, a dominant species in desert steppe, and the microorganisms in the roots, root epidermis, rhizosheath and rhizosphere soil were extracted and sequenced by 16S rRNA and ITS. The alpha diversity index of bacteria in Stipa grandis rhizosphere soil was the greatest, followed by rhizosheath, and the alpha diversity index of endophytic bacteria in root system was the smallest. The alpha diversity index of fungi in the rhizosheath and rhizosphere soil were significantly higher than that in the root epidermis and root system. There were significant differences in bacterial community structure between the root epidermis, endophytic bacteria, rhizosheath and rhizosphere soil. Unlike bacterial community structure, the community structure of fungi in the root epidermis was similar that of endophytic fungi, but significantly different from those in rhizosheath and rhizosphere soil. This study demonstrated a feasible method for separating plant rhizosheath and root epidermis. We suggest that the root epidermis can act as the interface between the host plant root and the external soil environment. We will have to re-examine the biological and ecological significance of rhizosheath and microorganisms in rhizosheath, as well as the mechanism explaining the close relationship of the rhizosheath and the plant root epidermis. This study provides theoretical and technical guidance for the isolation of the plant rhizosheath and the study of microorganisms in plant rhizosheath.
类型	Article
语种	英语
开放获取类型	gold, Green Published, Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000757537100024
WOS关键词	SYSTEMIC RESISTANCE ; SOIL-MICROORGANISMS ; SUMMER DORMANCY ; RHIZOSPHERE ; ARABIDOPSIS ; SEQUENCES
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376282
作者单位	[Zhu, Ai-Min; Wu, Qian; Liu, Hai-Li; Han, Guo-Dong] Inner Mongolia Agr Univ, Key Lab Forage Cultivat Proc & Higher Efficient U, Coll Grassland Resources & Environm,Inner Mongoli, Key Lab Grassland Resources,Minist Educ China,Min, Hohhot 010019, Peoples R China; [Sun, Hai-Lian] Res Base Acad Agr & Anim Husb Inner Mongolia, Hohhot 010031, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Ai-Min,Wu, Qian,Liu, Hai-Li,et al. Isolation of rhizosheath and analysis of microbial community structure around roots of Stipa grandis[J],2022,12(1).
APA	Zhu, Ai-Min,Wu, Qian,Liu, Hai-Li,Sun, Hai-Lian,&Han, Guo-Dong.(2022).Isolation of rhizosheath and analysis of microbial community structure around roots of Stipa grandis.SCIENTIFIC REPORTS,12(1).
MLA	Zhu, Ai-Min,et al."Isolation of rhizosheath and analysis of microbial community structure around roots of Stipa grandis".SCIENTIFIC REPORTS 12.1(2022).