干旱区生态环境知识资源中心(Arid): A miniaturized, low-cost lens tube based laser-induced fluorescence detection system for automated microfluidic analysis of primary amines

Arid

DOI	10.1016/j.talanta.2022.123227
	A miniaturized, low-cost lens tube based laser-induced fluorescence detection system for automated microfluidic analysis of primary amines
	Duca, Zachary A.; Speller, Nicholas C.; Cato, Michael E.; Morbioli, Giorgio Gianini; Stockton, Amanda M.
通讯作者	Stockton, AM (corresponding author)，901 Atlantic Dr,Rm,1102G, Atlanta, GA 30332 USA.
来源期刊	TALANTA
ISSN	0039-9140
EISSN	1873-3573
出版年	2022
卷号	241
英文摘要	In situ analyses are essential to ascertain potential past or present habitability in celestial bodies. One technique that provides the sensitivity and miniaturization needed to successfully detect trace organics in the outer Solar System is laser-induced fluorescence (LIF) detection, which, when coupled with microfluidic systems, provides a powerful wet chemistry platform that can meet the size and resource consumption constraints of a remote analysis mission. Herein, a portable LIF detection module (44-mm long, 18-mm wide) was prototyped and utilized to quantify bulk organics in a liquid sample via manual and automated analysis utilizing a programmable microfluidic architecture. The experimental limit of detection (LOD) for primary amines was 11.8 mu M. A sample (Y31B) collected from the Atacama Desert in Yungay, Chile, was analyzed manually and found to contain 300 +/- 50 mu M of bulk primary amine organics, while the automated microfluidic protocol found the sample to contain 289 +/- 4 mu M of primary amines. Automated analyses showed no statistically significant differences when compared to the manual analyses (t-test, C.I. 95%). Our results demonstrate that the coupling of programmable microfluidic devices with a custom lens tube-based LIF detector enables automated analysis of primary amines using a protocol appropriate for remote analyses. This technique is an invaluable tool for in situ analysis applications in distant, resource-restricted environments.
英文关键词	Laser-induced fluorescence detection Programmable microfluidic architectures Planetary science Fluorescamine dye Pacific blue dye Amines
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000759239600002
WOS关键词	ORGANIC-MOLECULES ; CARBONACEOUS BIOMARKERS ; ELECTROPHORESIS ; FLUORESCAMINE ; ACIDS ; INSTRUMENTATION ; DEVICES ; BIOLOGY
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376182
作者单位	[Duca, Zachary A.; Speller, Nicholas C.; Cato, Michael E.; Morbioli, Giorgio Gianini; Stockton, Amanda M.] Georgia Inst Technol, Atlanta, GA 30332 USA; [Duca, Zachary A.] Savannah River Natl Lab, Aiken, SC 29808 USA; [Speller, Nicholas C.; Cato, Michael E.] Georgia Tech Res Inst, Atlanta, GA 30318 USA; [Morbioli, Giorgio Gianini; Stockton, Amanda M.] Tufts Univ, Dept Chem, Medford, MA 02155 USA
推荐引用方式 GB/T 7714	Duca, Zachary A.,Speller, Nicholas C.,Cato, Michael E.,et al. A miniaturized, low-cost lens tube based laser-induced fluorescence detection system for automated microfluidic analysis of primary amines[J],2022,241.
APA	Duca, Zachary A.,Speller, Nicholas C.,Cato, Michael E.,Morbioli, Giorgio Gianini,&Stockton, Amanda M..(2022).A miniaturized, low-cost lens tube based laser-induced fluorescence detection system for automated microfluidic analysis of primary amines.TALANTA,241.
MLA	Duca, Zachary A.,et al."A miniaturized, low-cost lens tube based laser-induced fluorescence detection system for automated microfluidic analysis of primary amines".TALANTA 241(2022).