干旱区生态环境知识资源中心(Arid): PRE-TREATMENT WITH CYTOKININS IN Bouteloua curtipendula (MICHX.) TORR. TO INCREASE PLANTLET REGENERATION

Arid

	PRE-TREATMENT WITH CYTOKININS IN Bouteloua curtipendula (MICHX.) TORR. TO INCREASE PLANTLET REGENERATION
	Bernal-Flores, Alvaro; Perez-Rodriguez, Paulino; Robledo Paz, Alejandrina; Quero-Carrillo, Adrian R.
通讯作者	Quero-Carrillo, AR (corresponding author)，Colegio Postgraduados, Campus Montecillo, Texcoco, Estado De Mexic, Mexico.
来源期刊	REVISTA FITOTECNIA MEXICANA
ISSN	0187-7380
出版年	2021
卷号	44 期号:3
英文摘要	Sideoats grama [Bouteloua curtipendula (Michx.) Torr.] is one of the most widely cultivated forage species in semi-arid areas of the USA and Northern Mexico; it is an apomictic recalcitrant species with great forage attributes. The objective of this study was to evaluate pre-regeneration treatments using cytokinins to increase the frequency of regeneration. Induced morphogenic calli from mature caryopses of sideoats grama cv. El Reno cultured on basal Murashige and Skoog (MS) medium and supplemented with three levels of 2,4-dichlorophenoxyacetic acid (2,4-D) (2, 3 and 4 mg L-1) in presence of 0.15 mg L-1 of 6-benzylaminopurine (BAP) were subjected to two cytokinins-based treatments prior to the regeneration process [BAP (MSB) and BAP-KIN-TDZ (MSBKT)] for 10 days, and subsequently transferred to regeneration medium MSBK (2 mg L-1 BAP and 1 mg L-1 kinetin) and sub-cultured every 20 days until completing the regeneration process. Data were analyzed by logistic regression, negative binomial and mixed model. Statistical differences (P < 0.05) in callus induction frequency were recorded at different levels of 2,4-D, the highest being 2 mg L-1 showing the highest. Pretreatment based on MSB improved plantlet regeneration response as the level of 2,4-D increased in calli induction treatments, while lower levels increased the frequency of calli development. The highest regeneration response was observed in calli induced at 3 and 4 mg L-1 of 2,4-D (P < 0.05). Pretreatments with cytokinins proved to be a key factor for regeneration of sideoats grama cv El Reno plantlets.
英文关键词	Calli types plantlet regeneration recalcitrant apomictic sideoats grama cv. El Reno
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000740924600012
WOS关键词	SOMATIC EMBRYOGENESIS ; CALLUS-CULTURES ; TISSUE-CULTURE ; THIDIAZURON ; BARLEY ; INDUCTION ; AUXIN ; SEEDS ; GRASS
WOS类目	Agronomy ; Horticulture
WOS研究方向	Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/376018
作者单位	[Bernal-Flores, Alvaro; Perez-Rodriguez, Paulino; Robledo Paz, Alejandrina; Quero-Carrillo, Adrian R.] Colegio Postgraduados, Campus Montecillo, Texcoco, Estado De Mexic, Mexico; [Bernal-Flores, Alvaro] Semillas Papalotla SA CV, Area Invest & Desarrollo, Ocozocoautla, Chiapas, Mexico
推荐引用方式 GB/T 7714	Bernal-Flores, Alvaro,Perez-Rodriguez, Paulino,Robledo Paz, Alejandrina,et al. PRE-TREATMENT WITH CYTOKININS IN Bouteloua curtipendula (MICHX.) TORR. TO INCREASE PLANTLET REGENERATION[J],2021,44(3).
APA	Bernal-Flores, Alvaro,Perez-Rodriguez, Paulino,Robledo Paz, Alejandrina,&Quero-Carrillo, Adrian R..(2021).PRE-TREATMENT WITH CYTOKININS IN Bouteloua curtipendula (MICHX.) TORR. TO INCREASE PLANTLET REGENERATION.REVISTA FITOTECNIA MEXICANA,44(3).
MLA	Bernal-Flores, Alvaro,et al."PRE-TREATMENT WITH CYTOKININS IN Bouteloua curtipendula (MICHX.) TORR. TO INCREASE PLANTLET REGENERATION".REVISTA FITOTECNIA MEXICANA 44.3(2021).