干旱区生态环境知识资源中心(Arid): Venom of the desert black snake Walterinnesia aegyptia enhances anti-tumor immunity via its beneficial modulatory effects on pro- and anti-tumorigenic inflammatory mediators in cultured colon cancer cells

Arid

DOI	10.1093/toxres/tfab093
	Venom of the desert black snake Walterinnesia aegyptia enhances anti-tumor immunity via its beneficial modulatory effects on pro- and anti-tumorigenic inflammatory mediators in cultured colon cancer cells
	Daghestani, Maha H.; Ambreen, Khushboo; Hakami, Hana H.; Omair, Mohammed A.; Saleem, Abdulaziz M.; Aleisa, Nadia A.; AlNeghery, Lina M.; Amin, Mohannad H.; Alobaid, Hussah M.; Omair, Maha A.; Hassen, Lena M.
通讯作者	Ambreen, K (corresponding author)，Integral Univ, Dept Biotechnol, Lucknow 226026, Uttar Pradesh, India.
来源期刊	TOXICOLOGY RESEARCH
ISSN	2045-452X
EISSN	2045-4538
出版年	2021
卷号	10 期号:6
英文摘要	The role of inflammation in colon cancer is understood as a well-accepted factor that has the tendency to release multiple pro- and anti-tumorigenic inflammatory mediators. Inflammation-induced increased expression of anti-tumorigenic inflammatory mediators and decreased expression of pro-tumorigenic inflammatory mediators encourage beneficial inflammatory effects in terms of powerful anti-tumor immunity. The present study aims to screen the beneficial inflammatory effects of Walterinnesia aegyptia venom via determining its modulatory tendency on the expression of 40 pro- and anti-tumorigenic inflammatory mediators (cytokines/growth factors/chemokines) in LoVo human colon cancer cell line. LoVo-cells were treated with varying doses of crude venom of W. aegyptia. Cell viability was checked utilizing flow cytometry, and IC50 of venom was determined. Venom-induced inflammatory effects were evaluated on the expression of 40 different inflammatory mediators (12 anti-tumorigenic cytokines, 11 pro-tumorigenic cytokines, 7 pro-tumorigenic growth factors, 9 pro-tumorigenic chemokines and 1 anti-tumorigenic chemokine) in treated LoVo-cells [utilizing enzyme-linked immunosorbent assay (ELISA)] and compared with controls. Treatment of venom induced significant cytotoxic effects on inflamed LoVo-cells. IC50 treatment of venom caused significant modulations on the expression of 22 inflammatory mediators in treated LoVo-cells. The beneficial modulatory effects of venom were screened via its capability to significantly increase the expression of five powerful anti-tumorigenic mediators (IL-9, IL-12p40, IL-15, IL-1RA and Fractalkine) and decrease the expression of four major pro-tumorigenic mediators (IL-1 beta, VEGF, MCP-1 and MCP-3). Walterinnesia aegyptia venom-induced beneficial modulations on the expression of nine crucial pro/anti-tumorigenic inflammatory mediators can be effectively used to enhance powerful anti-tumor immunity against colon cancer.
英文关键词	colon cancerWalterinnesia aegyptia venominflammationanti-tumor immunitycytokinesgrowth factorschemokines
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000734651000005
WOS关键词	COLORECTAL-CANCER ; GROWTH ARREST ; CYTOKINES ; APOPTOSIS ; PROGRESSION ; METASTASIS ; EXPRESSION ; CROSSTALK ; PATHWAYS ; CCL7
WOS类目	Toxicology
WOS研究方向	Toxicology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/375891
作者单位	[Daghestani, Maha H.; Hakami, Hana H.; Aleisa, Nadia A.; Alobaid, Hussah M.; Hassen, Lena M.] King Saud Univ, Dept Zool, Coll Sci, Ctr Sci & Med Female Coll, Riyadh, Saudi Arabia; [Ambreen, Khushboo] Integral Univ, Dept Biotechnol, Lucknow 226026, Uttar Pradesh, India; [Omair, Mohammed A.] King Saud Univ, Dept Med, Div Rheumatol, Riyadh, Saudi Arabia; [Saleem, Abdulaziz M.] King Abdulaziz Univ, Med Coll, Dept Surg, Jeddah, Saudi Arabia; [AlNeghery, Lina M.] Al Imam Mohammad Ibn Saud Islamic Univ, Coll Sci, Dept Biol, Riyadh, Saudi Arabia; [Amin, Mohannad H.] Riyadh ELM Univ, Coll Dent, Riyadh, Saudi Arabia; [Omair, Maha A.] King Saud Univ, Coll Sci, Dept Stat & Operat Res, Riyadh, Saudi Arabia
推荐引用方式 GB/T 7714	Daghestani, Maha H.,Ambreen, Khushboo,Hakami, Hana H.,et al. Venom of the desert black snake Walterinnesia aegyptia enhances anti-tumor immunity via its beneficial modulatory effects on pro- and anti-tumorigenic inflammatory mediators in cultured colon cancer cells[J],2021,10(6).
APA	Daghestani, Maha H..,Ambreen, Khushboo.,Hakami, Hana H..,Omair, Mohammed A..,Saleem, Abdulaziz M..,...&Hassen, Lena M..(2021).Venom of the desert black snake Walterinnesia aegyptia enhances anti-tumor immunity via its beneficial modulatory effects on pro- and anti-tumorigenic inflammatory mediators in cultured colon cancer cells.TOXICOLOGY RESEARCH,10(6).
MLA	Daghestani, Maha H.,et al."Venom of the desert black snake Walterinnesia aegyptia enhances anti-tumor immunity via its beneficial modulatory effects on pro- and anti-tumorigenic inflammatory mediators in cultured colon cancer cells".TOXICOLOGY RESEARCH 10.6(2021).