干旱区生态环境知识资源中心(Arid): Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants

Arid

DOI	10.3389/fpls.2021.744699
	Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants
	Ahmad, Hasan; Maher, Mohamed; Abdel-Salam, Eslam M.; Li, Yufei; Yang, Chenkun; ElSafty, Nagwa; Ewas, Mohamed; Nishawy, Elsayed; Luo, Jie
通讯作者	Luo, J (corresponding author)，Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China. ; Luo, J (corresponding author)，Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan, Peoples R China. ; Luo, J (corresponding author)，Hainan Univ, Coll Trop Crops, Haikou, Hainan, Peoples R China.
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2021
卷号	12
英文摘要	Solenostemma argel (Delile) Hayne is a desert plant that survives harsh environmental conditions with several vital medicinal properties. Salt stress is a major constraint limiting agricultural production around the globe. However, response mechanisms behind the adaptation of S. argel plants to salt stress are still poorly understood. In the current study, we applied an omics approach to explore how this plant adapts to salt stress by integrating transcriptomic and metabolomic changes in the roots and leaves of S. argel plants under salt stress. De novo assembly of transcriptome produced 57,796 unigenes represented by 165,147 transcripts/isoforms. A total of 730 differentially expressed genes (DEGs) were identified in the roots (396 and 334 were up- and down-regulated, respectively). In the leaves, 927 DEGs were identified (601 and 326 were up- and down-regulated, respectively). Gene ontology and Kyoto Encyclopedia of Genes And Genomes pathway enrichment analyses revealed that several defense-related biological processes, such as response to osmotic and oxidative stress, hormonal signal transduction, mitogen-activated protein kinase signaling, and phenylpropanoid biosynthesis pathways are the potential mechanisms involved in the tolerance of S. argel plants to salt stress. Furthermore, liquid chromatography-tandem mass spectrometry was used to detect the metabolic variations of the leaves and roots of S. argel under control and salt stress. 45 and 56 critical metabolites showed changes in their levels in the stressed roots and leaves, respectively; there were 20 metabolites in common between the roots and leaves. Differentially accumulated metabolites included amino acids, polyamines, hydroxycinnamic acids, monolignols, flavonoids, and saccharides that improve antioxidant ability and osmotic adjustment of S. argel plants under salt stress. The results present insights into potential salt response mechanisms in S. argel desert plants and increase the knowledge in order to generate more tolerant crops to salt stress.
英文关键词	transcriptome metabolome phenylpropanoid salt stress Solenostemma argel
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000742468300001
WOS关键词	GENOME-WIDE ASSOCIATION ; STRESS TOLERANCE ; DROUGHT STRESS ; ENHANCED SALT ; ARABIDOPSIS ; ACCUMULATION ; PROLINE ; PROTEIN ; OVEREXPRESSION ; IDENTIFICATION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/375840
作者单位	[Ahmad, Hasan; Maher, Mohamed; Li, Yufei; Yang, Chenkun; Ewas, Mohamed; Nishawy, Elsayed; Luo, Jie] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China; [Ahmad, Hasan; Maher, Mohamed; Li, Yufei; Yang, Chenkun; Ewas, Mohamed; Nishawy, Elsayed; Luo, Jie] Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan, Peoples R China; [Ahmad, Hasan] Agr Res Ctr, Natl Gene Bank, Giza, Egypt; [Maher, Mohamed] Zagazig Univ, Fac Agr, Biochem Dept, Zagazig, Egypt; [Abdel-Salam, Eslam M.] King Saud Univ, Dept Bot & Microbiol, Coll Sci, Riyadh, Saudi Arabia; [ElSafty, Nagwa; Ewas, Mohamed; Nishawy, Elsayed] Desert Res Ctr, Plant Genet Resources Dept, Cairo, Egypt; [Luo, Jie] Hainan Univ, Coll Trop Crops, Haikou, Hainan, Peoples R China
推荐引用方式 GB/T 7714	Ahmad, Hasan,Maher, Mohamed,Abdel-Salam, Eslam M.,et al. Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants[J],2021,12.
APA	Ahmad, Hasan.,Maher, Mohamed.,Abdel-Salam, Eslam M..,Li, Yufei.,Yang, Chenkun.,...&Luo, Jie.(2021).Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants.FRONTIERS IN PLANT SCIENCE,12.
MLA	Ahmad, Hasan,et al."Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants".FRONTIERS IN PLANT SCIENCE 12(2021).