干旱区生态环境知识资源中心(Arid): Characterization of a Chickpea Mutant Resistant to Phelipanche aegyptiaca Pers. and Orobanche crenata Forsk

Arid

DOI	10.3390/plants10122552
	Characterization of a Chickpea Mutant Resistant to Phelipanche aegyptiaca Pers. and Orobanche crenata Forsk
	Galili, Shmuel; Hershenhorn, Joseph; Smirnov, Evgeny; Yoneyama, Koichi; Xie, Xiaonan; Amir-Segev, Orit; Bellalou, Aharon; Dor, Evgenia
通讯作者	Galili, S (corresponding author)，Agr Res Org, Inst Plant Sci, Volcani Ctr, POB 15159,HaMaccabim Rd 68, IL-7505101 Rishon Leziyyon, Israel. ; Dor, E (corresponding author)，Agr Res Org, Inst Plant Protect, Newe Yaar Res Ctr, POB 1021, IL-3009503 Ramat Yishay, Israel.
来源期刊	PLANTS-BASEL
EISSN	2223-7747
出版年	2021
卷号	10 期号:12
英文摘要	Chickpea (Cicer arietinum L.) is a major pulse crop in Israel grown on about 3000 ha spread, from the Upper Galilee in the north to the North-Negev desert in the south. In the last few years, there has been a gradual increase in broomrape infestation in chickpea fields in all regions of Israel. Resistant chickpea cultivars would be simple and effective solution to control broomrape. Thus, to develop resistant cultivars we screened an ethyl methanesulfonate (EMS) mutant population of F01 variety (Kabuli type) for broomrape resistance. One of the mutant lines (CCD7M14) was found to be highly resistant to both Phelipanche aegyptiaca and Orobanche crenata. The resistance mechanism is based on the inability of the mutant to produce strigolactones (SLs)-stimulants of broomrape seed germination. LC/MS/MS analysis revealed the SLs orobanchol, orobanchyl acetate, and didehydroorobanchol in root exudates of the wild type, but no SLs could be detected in the root exudates of CCD7M14. Sequence analyses revealed a point mutation (G-to-A transition at nucleotide position 210) in the Carotenoid Cleavage Dioxygenase 7 (CCD7) gene that is responsible for the production of key enzymes in the biosynthesis of SLs. This nonsense mutation resulted in a CCD7 stop codon at position 70 of the protein. The influences of the CCD7M14 mutation on chickpea phenotype and chlorophyll, carotenoid, and anthocyanin content were characterized.
英文关键词	chickpea strigolactone broomrape resistance chickpea phenotype chlorophyll carotenoid anthocyanin
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000738188100001
WOS关键词	STRIGOLACTONE BIOSYNTHESIS ; GERMINATION STIMULANTS ; BROOMRAPE OROBANCHE ; LEAF SENESCENCE ; PARASITIC WEEDS ; SHOOT ; ARABIDOPSIS ; INHIBITION ; CARLACTONE ; STRIGA
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/375766
作者单位	[Galili, Shmuel; Amir-Segev, Orit; Bellalou, Aharon] Agr Res Org, Inst Plant Sci, Volcani Ctr, POB 15159,HaMaccabim Rd 68, IL-7505101 Rishon Leziyyon, Israel; [Hershenhorn, Joseph; Smirnov, Evgeny; Dor, Evgenia] Agr Res Org, Inst Plant Protect, Newe Yaar Res Ctr, POB 1021, IL-3009503 Ramat Yishay, Israel; [Yoneyama, Koichi; Xie, Xiaonan] Utsunomiya Univ, Ctr Biosci Res & Educ, 350 Mine Machi, Utsunomiya, Tochigi 3218505, Japan
推荐引用方式 GB/T 7714	Galili, Shmuel,Hershenhorn, Joseph,Smirnov, Evgeny,et al. Characterization of a Chickpea Mutant Resistant to Phelipanche aegyptiaca Pers. and Orobanche crenata Forsk[J],2021,10(12).
APA	Galili, Shmuel.,Hershenhorn, Joseph.,Smirnov, Evgeny.,Yoneyama, Koichi.,Xie, Xiaonan.,...&Dor, Evgenia.(2021).Characterization of a Chickpea Mutant Resistant to Phelipanche aegyptiaca Pers. and Orobanche crenata Forsk.PLANTS-BASEL,10(12).
MLA	Galili, Shmuel,et al."Characterization of a Chickpea Mutant Resistant to Phelipanche aegyptiaca Pers. and Orobanche crenata Forsk".PLANTS-BASEL 10.12(2021).