Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.3390/ijms222413486 |
| A Genus-Wide Bioactivity Analysis of Daboia (Viperinae: Viperidae) Viper Venoms Reveals Widespread Variation in Haemotoxic Properties | |
| op den Brouw, Bianca; Coimbra, Francisco C. P.; Casewell, Nicholas R.; Ali, Syed Abid; Vonk, Freek J.; Fry, Bryan G. | |
| 通讯作者 | op den Brouw, B ; Fry, BG (corresponding author)，Univ Queensland, Sch Biol Sci, Venom Evolut Lab, St Lucia, Qld 4072, Australia. |
| 来源期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| EISSN | 1422-0067 |
| 出版年 | 2021 |
| 卷号 | 22期号:24 |
| 英文摘要 | The snake genus Daboia (Viperidae: Viperinae; Oppel, 1811) contains five species: D. deserti, D. mauritanica, and D. palaestinae, found in Afro-Arabia, and the Russell's vipers D. russelii and D. siamensis, found in Asia. Russell's vipers are responsible for a major proportion of the medically important snakebites that occur in the regions they inhabit, and their venoms are notorious for their coagulopathic effects. While widely documented, the extent of venom variation within the Russell's vipers is poorly characterised, as is the venom activity of other species within the genus. In this study we investigated variation in the haemotoxic activity of Daboia using twelve venoms from all five species, including multiple variants of D. russelii, D. siamensis, and D. palaestinae. We tested the venoms on human plasma using thromboelastography, dose-response coagulometry analyses, and calibrated automated thrombography, and on human fibrinogen by thromboelastography and fibrinogen gels. We assessed activation of blood factors X and prothrombin by the venoms using fluorometry. Variation in venom activity was evident in all experiments. The Asian species D. russelii and D. siamensis and the African species D. mauritanica possessed procoagulant venom, while D. deserti and D. palaestinae were net-anticoagulant. Of the Russell's vipers, the venom of D. siamensis from Myanmar was most toxic and D. russelli of Sri Lanka the least. Activation of both factor X and prothrombin was evident by all venoms, though at differential levels. Fibrinogenolytic activity varied extensively throughout the genus and followed no phylogenetic trends. This venom variability underpins one of the many challenges facing treatment of Daboia snakebite envenoming. Comprehensive analyses of available antivenoms in neutralising these variable venom activities are therefore of utmost importance. |
| 英文关键词 | Daboia Russell's viper venom toxin venom variation haemotoxicity coagulopathy fibrinogenolysis snakebite envenoming |
| 类型 | Article |
| 语种 | 英语 |
| 开放获取类型 | Green Published |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000738557600001 |
| WOS关键词 | MACROVIPERA-MAURITANICA ; SNAKE-VENOM ; FUNCTIONAL VENOMICS ; THROMBIN GENERATION ; RUSSELII VENOM ; PROTHROMBIN ; FIBRIN ; METALLOPROTEASE ; HEMOSTASIS ; POLYVALENT |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/375752 |
| 作者单位 | [op den Brouw, Bianca; Coimbra, Francisco C. P.; Fry, Bryan G.] Univ Queensland, Sch Biol Sci, Venom Evolut Lab, St Lucia, Qld 4072, Australia; [Casewell, Nicholas R.] Univ Liverpool Liverpool Sch Trop Med, Ctr Snakebite Res & Intervent, Liverpool L3 5QA, Merseyside, England; [Ali, Syed Abid] Univ Karachi, HEJ Res Inst Chem, World Ctr Sci & Technol 3, Karachi 75270, Pakistan; [Vonk, Freek J.] Naturalis Biodivers Ctr, NL-2333 CR Leiden, Netherlands; [Vonk, Freek J.] Vrije Univ Amsterdam, Amsterdam Inst Mol & Life Sci, Div BioAnalyt Chem, Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands |
| 推荐引用方式 GB/T 7714 | op den Brouw, Bianca,Coimbra, Francisco C. P.,Casewell, Nicholas R.,et al. A Genus-Wide Bioactivity Analysis of Daboia (Viperinae: Viperidae) Viper Venoms Reveals Widespread Variation in Haemotoxic Properties[J],2021,22(24). |
| APA | op den Brouw, Bianca,Coimbra, Francisco C. P.,Casewell, Nicholas R.,Ali, Syed Abid,Vonk, Freek J.,&Fry, Bryan G..(2021).A Genus-Wide Bioactivity Analysis of Daboia (Viperinae: Viperidae) Viper Venoms Reveals Widespread Variation in Haemotoxic Properties.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(24). |
| MLA | op den Brouw, Bianca,et al."A Genus-Wide Bioactivity Analysis of Daboia (Viperinae: Viperidae) Viper Venoms Reveals Widespread Variation in Haemotoxic Properties".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.24(2021). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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