干旱区生态环境知识资源中心(Arid): Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma

Arid

DOI	10.3389/fcell.2021.793638
	Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma
	Wang, Liyuan; Yu, Tianyang; Zhang, Xiaohui; Cai, Xiaojun; Sun, He
通讯作者	Sun, H (corresponding author)，Heilongjiang Univ Chinese Med, Dept Ophthalmol, Affiliated Hosp 1, Harbin, Peoples R China. ; Cai, XJ (corresponding author)，Heilongjiang Acad Sci Tradit Chinese Med, Dept Endocrinol, Harbin, Peoples R China.
来源期刊	FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
ISSN	2296-634X
出版年	2021
卷号	9
英文摘要	Primary open-angle glaucoma (POAG) is a progressive optic neuropathy and its damage to vision is irreversible. Therefore, early diagnosis assisted by biomarkers is essential. Although there were multiple researches on the identification of POAG biomarkers, few studies systematically revealed the transcriptome dysregulation mechanism of POAG from the perspective of pre- and post-transcription of genes. Here, we have collected multiple sets of POAG's aqueous humor (AH) tissue transcription profiles covering long non-coding RNA (lncRNA), mRNA and mircoRNA (miRNA). Through differential expression analysis, we identified thousands of significant differentially expressed genes (DEGs) between the AH tissue of POAG and non-glaucoma. Further, the DEGs were used to construct a competing endogenous RNA (ceRNA) regulatory network and 1,653 qualified lncRNA-miRNA-mRNA regulatory units were identified. Two ceRNA regulatory subnets were identified based on the random walk algorithm and revealed to be involved in the regulation of multiple complex diseases. At the pre-transcriptional regulation level, a transcriptional regulatory network was constructed and three transcription factors (FOS, ATF4, and RELB) were identified to regulate the expression of multiple genes and participate in the regulation of T cells. Moreover, we revealed the immune desert status of AH tissue for POAG patients based on immune infiltration analysis and identified a specific AL590666.2-hsa-miR-339-5p-UROD axis can be used as a biomarker of POAG. Taken together, the identification of regulatory mechanisms and biomarkers will contribute to the individualized diagnosis and treatment for POAG.
英文关键词	primary open-angle glaucoma ceRNA transcription factors immune infiltration biomarkers
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000760981700001
WOS关键词	PROLIFERATION ; SUPPRESSION ; RESOURCE ; GENES ; CERNA
WOS类目	Cell Biology ; Developmental Biology
WOS研究方向	Cell Biology ; Developmental Biology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/375695
作者单位	[Wang, Liyuan; Sun, He] Heilongjiang Univ Chinese Med, Dept Ophthalmol, Affiliated Hosp 1, Harbin, Peoples R China; [Yu, Tianyang] Heilongjiang Univ Chinese Med, Dept Acupuncture, Affiliated Hosp 2, Harbin, Peoples R China; [Zhang, Xiaohui] Heilongjiang Prov Eye Hosp, Dept Ophthalmol, Harbin, Peoples R China; [Cai, Xiaojun] Heilongjiang Acad Sci Tradit Chinese Med, Dept Endocrinol, Harbin, Peoples R China
推荐引用方式 GB/T 7714	Wang, Liyuan,Yu, Tianyang,Zhang, Xiaohui,et al. Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma[J],2021,9.
APA	Wang, Liyuan,Yu, Tianyang,Zhang, Xiaohui,Cai, Xiaojun,&Sun, He.(2021).Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma.FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,9.
MLA	Wang, Liyuan,et al."Network Integration Analysis and Immune Infiltration Analysis Reveal Potential Biomarkers for Primary Open-Angle Glaucoma".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9(2021).