干旱区生态环境知识资源中心(Arid): A Round Trip to the Desert: In situ Nanopore Sequencing Informs Targeted Bioprospecting

Arid

DOI	10.3389/fmicb.2021.768240
	A Round Trip to the Desert: In situ Nanopore Sequencing Informs Targeted Bioprospecting
	Latorre-Perez, Adriel; Gimeno-Valero, Helena; Tanner, Kristie; Pascual, Javier; Vilanova, Cristina; Porcar, Manuel
通讯作者	Vilanova, C (corresponding author)，Darwin Bioprospecting Excellence SL, Darwin, NT, Spain.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2021
卷号	12
英文摘要	Bioprospecting expeditions are often performed in remote locations, in order to access previously unexplored samples. Nevertheless, the actual potential of those samples is only assessed once scientists are back in the laboratory, where a time-consuming screening must take place. This work evaluates the suitability of using Nanopore sequencing during a journey to the Tabernas Desert (Spain) for forecasting the potential of specific samples in terms of bacterial diversity and prevalence of radiation- and desiccation-resistant taxa, which were the target of the bioprospecting activities. Samples collected during the first day were analyzed through 16S rRNA gene sequencing using a mobile laboratory. Results enabled the identification of locations showing the greatest and the least potential, and a second, informed sampling was performed focusing on those sites. After finishing the expedition, a culture collection of 166 strains belonging to 50 different genera was established. Overall, Nanopore and culturing data correlated well, since samples holding a greater potential at the microbiome level also yielded a more interesting set of microbial isolates, whereas samples showing less biodiversity resulted in a reduced (and redundant) set of culturable bacteria. Thus, we anticipate that portable sequencers hold potential as key, easy-to-use tools for in situ-informed bioprospecting strategies.
英文关键词	Nanopore sequencing bioprospecting in situ sequencing 16S rRNA gene sequencing microbiome analysis Tabernas Desert
类型	Article
语种	英语
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000735974100001
WOS关键词	SP NOV. ; SOIL ; DIVERSITY ; BACTERIA
WOS类目	Microbiology
WOS研究方向	Microbiology
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/375682
作者单位	[Latorre-Perez, Adriel; Gimeno-Valero, Helena; Tanner, Kristie; Pascual, Javier; Vilanova, Cristina; Porcar, Manuel] Darwin Bioprospecting Excellence SL, Darwin, NT, Spain; [Porcar, Manuel] Inst Integrat Syst Biol I2SysBio Univ Valencia CS, Paterna, Spain
推荐引用方式 GB/T 7714	Latorre-Perez, Adriel,Gimeno-Valero, Helena,Tanner, Kristie,et al. A Round Trip to the Desert: In situ Nanopore Sequencing Informs Targeted Bioprospecting[J],2021,12.
APA	Latorre-Perez, Adriel,Gimeno-Valero, Helena,Tanner, Kristie,Pascual, Javier,Vilanova, Cristina,&Porcar, Manuel.(2021).A Round Trip to the Desert: In situ Nanopore Sequencing Informs Targeted Bioprospecting.FRONTIERS IN MICROBIOLOGY,12.
MLA	Latorre-Perez, Adriel,et al."A Round Trip to the Desert: In situ Nanopore Sequencing Informs Targeted Bioprospecting".FRONTIERS IN MICROBIOLOGY 12(2021).