干旱区生态环境知识资源中心(Arid): AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants

Arid

DOI	10.3389/fpls.2021.788938
	AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants
	Sun, Yibo; Liu, Linghao; Sun, Shaokun; Han, Wangzhen; Irfan, Muhammad; Zhang, Xiaojia; Zhang, Li; Chen, Lijing
通讯作者	Zhang, L ; Chen, LJ (corresponding author)，Shenyang Agr Univ, Coll Biosci & Biotechnol, Key Lab Agr Biotechnol Liaoning Prov, Shenyang, Peoples R China. ; Chen, LJ (corresponding author)，Shenyang Agr Univ, Minist Educ, Coll Hort, Key Lab Protected Hort, Shenyang, Peoples R China.
来源期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
出版年	2021
卷号	12
英文摘要	Dehydrins (DHNs) play crucial roles in a broad spectrum of abiotic stresses in model plants. However, the evolutionary role of DHNs has not been explored, and the function of DHN proteins is largely unknown in Ammopiptanthus nanus (A. nanus), an ancient and endangered legume species from the deserts of northwestern China. In this study, we isolated a drought-response gene (c195333_g1_i1) from a drought-induced RNA-seq library of A. nanus. Evolutionary bioinformatics showed that c195333_g1_i1 is an ortholog of Arabidopsis DHN, and we renamed it AnDHN. Moreover, DHN proteins may define a class of proteins that are evolutionarily conserved in all angiosperms that have experienced a contraction during the evolution of legumes. Arabidopsis plants overexpressing AnDHN exhibited morpho-physiological changes, such as an increased germination rate, higher relative water content (RWC), higher proline (PRO) content, increased peroxidase (POD) and catalase (CAT) activities, lower contents of malondialdehyde (MDA), H2O2 and O-2(-), and longer root length. Our results showed that the transgenic lines had improved drought resistance with deep root system architecture, excellent water retention, increased osmotic adjustment, and enhanced reactive oxygen species (ROS) scavenging. Furthermore, the transgenic lines also had enhanced salt and cold tolerance. Our findings demonstrate that AnDHN may be a good candidate gene for improving abiotic stress tolerance in crops.Key Message: Using transcriptome analysis in Ammopiptanthus nanus, we isolated a drought-responsive gene, AnDHN, that plays a key role in enhancing abiotic stress tolerance in plants, with strong functional diversification in legumes.
英文关键词	Ammopiptanthus nanus drought stress abiotic stress legume AnDHN ROS
类型	Article
语种	英语
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000743516400001
WOS关键词	ENHANCES TOLERANCE ; COLD-ACCLIMATION ; SK3-TYPE DEHYDRIN ; FREEZING-TOLERANCE ; ABIOTIC STRESSES ; IMPROVES DROUGHT ; OSMOTIC-STRESS ; IN-VITRO ; GENE ; OVEREXPRESSION
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/375657
作者单位	[Sun, Yibo; Liu, Linghao; Han, Wangzhen; Zhang, Xiaojia; Zhang, Li; Chen, Lijing] Shenyang Agr Univ, Coll Biosci & Biotechnol, Key Lab Agr Biotechnol Liaoning Prov, Shenyang, Peoples R China; [Sun, Yibo; Sun, Shaokun; Chen, Lijing] Shenyang Agr Univ, Minist Educ, Coll Hort, Key Lab Protected Hort, Shenyang, Peoples R China; [Irfan, Muhammad] Univ Sargodha, Fac Sci, Dept Biotechnol, Sargodha, Pakistan
推荐引用方式 GB/T 7714	Sun, Yibo,Liu, Linghao,Sun, Shaokun,et al. AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants[J],2021,12.
APA	Sun, Yibo.,Liu, Linghao.,Sun, Shaokun.,Han, Wangzhen.,Irfan, Muhammad.,...&Chen, Lijing.(2021).AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants.FRONTIERS IN PLANT SCIENCE,12.
MLA	Sun, Yibo,et al."AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants".FRONTIERS IN PLANT SCIENCE 12(2021).