干旱区生态环境知识资源中心(Arid): Alteration of proteome in germinating seedlings of piegonpea (Cajanus cajan) after salt stress

Arid

DOI	10.1007/s12298-021-01116-w
	Alteration of proteome in germinating seedlings of piegonpea (Cajanus cajan) after salt stress
	Jain, Neha; Farhat, Sufia; Kumar, Ram; Singh, Nisha; Singh, Sangeeta; Sreevathsa, Rohini; Kalia, Sanjay; Singh, Nagendra Kumar; Teruhiro, Takabe; Rai, Vandna
通讯作者	Rai, V (corresponding author), ICAR Natl Inst Plant Biotechnol, Lal Bahadur Shastri Bldg,Pusa Rd, New Delhi 110012, India.
来源期刊	PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
ISSN	0971-5894
EISSN	0974-0430
出版年	2021
英文摘要	Pigeonpea (Cajanus cajan) is an important crop in semi-arid regions and a significant source of dietary proteins in India. The plant is sensitive to salinity stress, which adversely affects its productivity. Based on the dosage-dependent influence of salinity stress on the growth and ion contents in the young seedlings of pigeonpea, a comparative proteome analysis of control and salt stressed (150 mM NaCl) plants was conducted using 7 days-old seedlings. Among various amino acids, serine, aspartate and asparagine were the amino acids that showed increment in the root, whereas serine, aspartate and phenylalanine showed an upward trend in shoots under salt stress. Furthermore, a label-free and gel-free comparative Q-Tof, Liquid Chromatography-Mass spectrometry (LC-MS) revealed total of 118 differentially abundant proteins in roots and shoots with and without salt stress conditions. Proteins related to DNA-binding with one finger (Dof) transcription factor family and glycine betaine (GB) biosynthesis were differentially expressed in the shoot and root of the salinity-stressed seedlings. Exogenous application of choline on GB accumulation under salt stress showed the increase of GB pathway in C. cajan. Gene expression analysis for differentially abundant proteins revealed the higher induction of ethanolamine kinase (CcEthKin), choline-phosphate cytidylyltransferase 1-like (CcChoPh), serine hydroxymethyltransferase (CcSHMT) and Dof protein (CcDof29). The results indicate the importance of, choline precursor, serine biosynthetic pathways and glycine betaine synthesis in salinity stress tolerance. The glycine betaine protects plant from cellular damages and acts as osmoticum under stress condition. Protein interaction network (PIN) analysis demonstrated that 61% of the differentially expressed proteins exhibited positive interactions and 10% of them formed the center of the PIN. Further, The PIN analysis also highlighted the potential roles of the cytochrome c oxidases in sensing and signaling cascades governing salinity stress responses in pigeonpea.
英文关键词	Pigeonpea Q-Tof Proteins Salinity Glycine betaine
类型	Article
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000733229200003
WOS关键词	CHOLINE MONOOXYGENASE ; FUNCTIONAL-CHARACTERIZATION ; GLYCINE BETAINE ; SALINITY STRESS ; SUGAR-BEET ; ACCUMULATION ; EXPRESSION ; TOLERANCE ; SPINACH ; DROUGHT
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374740
作者单位	[Jain, Neha; Farhat, Sufia; Kumar, Ram; Singh, Nisha; Singh, Sangeeta; Sreevathsa, Rohini; Singh, Nagendra Kumar; Rai, Vandna] ICAR Natl Inst Plant Biotechnol, Lal Bahadur Shastri Bldg,Pusa Rd, New Delhi 110012, India; [Farhat, Sufia] IK Gujral Punjab Tech Univ, Jalandhar, Punjab, India; [Kalia, Sanjay] Dept Biotechnol, New Delhi, India; [Teruhiro, Takabe] Meijo Univ, Res Inst, Nagoya, Aichi 4688502, Japan
推荐引用方式 GB/T 7714	Jain, Neha,Farhat, Sufia,Kumar, Ram,et al. Alteration of proteome in germinating seedlings of piegonpea (Cajanus cajan) after salt stress[J],2021.
APA	Jain, Neha.,Farhat, Sufia.,Kumar, Ram.,Singh, Nisha.,Singh, Sangeeta.,...&Rai, Vandna.(2021).Alteration of proteome in germinating seedlings of piegonpea (Cajanus cajan) after salt stress.PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS.
MLA	Jain, Neha,et al."Alteration of proteome in germinating seedlings of piegonpea (Cajanus cajan) after salt stress".PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS (2021).