干旱区生态环境知识资源中心(Arid): Morphological and molecular markers based assessment of genetic diversity of a valuable endemic plant Lasiurus sindicus Henr. in the arid region of Rajasthan, India

Arid

DOI	10.1080/15324982.2021.2012728
	Morphological and molecular markers based assessment of genetic diversity of a valuable endemic plant Lasiurus sindicus Henr. in the arid region of Rajasthan, India
	Goswami, Bhuwnesh; Gadi, B. R.; Rao, Satyawada Rama
通讯作者	Gadi, BR (corresponding author), Jai Narain Vyas Univ, Ctr Adv Study CAS, Dept Bot, Stress Physiol & Mol Biol Lab, Jodhpur, Rajasthan, India.
来源期刊	ARID LAND RESEARCH AND MANAGEMENT
ISSN	1532-4982
EISSN	1532-4990
出版年	2021-12
英文摘要	Morphological and molecular markers are useful in genetic diversity assessment and improvement of plants. In the present study, five morphological traits, random amplified polymorphic DNA (RAPD) and Start codon targeted (SCoT) markers were used for genetic diversity evaluation of Sewan grass (Lasiurus sindicus Henr.), a valuable endemic plant of Indian Thar desert. Accessions collected from 14 different sites were used for measuring morphological traits, namely plant height (PH), number of tillers (NT), number of nodes (NN), leaf length (LL) and spike length (SL) which showed large variations among populations; particularly PH, NT and LL. Ward's linkage hierarchical cluster analysis exhibited two major clusters and Cluster I contained two minor clusters. The percentage polymorphic bands (PPB) produced were 89.64% and 64.51% for RAPD and SCoT respectively. RAPD markers based dendrogram revealed three clades while there was no clear clade formation with SCoT markers. Single primer amplification reaction (SPAR) analysis with combined data of RAPD + SCoT showed more similarity with SCoT than with RAPD clusters. AMOVA revealed higher variation within population (90%) than among populations (10%) of L. sindicus. The Principal coordinate analysis (PCoA) also supported this result. There was no correlation between morphological and molecular data confirming environmental impact on morphological variation. SCoT and SPAR markers have clearly identified a novel genotype (LS JS 3) which has high values of PH, NN and LL. This study suggests that morphological data combined with molecular data can alone help in the identification of novel genotypes of L. sindicus among its populations.
英文关键词	Cluster analysis conservation genetics Lasiurus sindicus Henr molecular markers morphological traits
类型	Article ; Early Access
语种	英语
收录类别	SCI-E
WOS记录号	WOS:000730045200001
WOS关键词	CHOLISTAN DESERT ; AFLP MARKERS ; HENERD
WOS类目	Environmental Sciences ; Soil Science
WOS研究方向	Environmental Sciences & Ecology ; Agriculture
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374710
作者单位	[Goswami, Bhuwnesh; Gadi, B. R.] Jai Narain Vyas Univ, Ctr Adv Study CAS, Dept Bot, Stress Physiol & Mol Biol Lab, Jodhpur, Rajasthan, India; [Rao, Satyawada Rama] North Eastern Hill Univ, Plant Biotechnol Lab, Dept Biotechnol & Bioinformat, Shillong, Meghalaya, India
推荐引用方式 GB/T 7714	Goswami, Bhuwnesh,Gadi, B. R.,Rao, Satyawada Rama. Morphological and molecular markers based assessment of genetic diversity of a valuable endemic plant Lasiurus sindicus Henr. in the arid region of Rajasthan, India[J],2021.
APA	Goswami, Bhuwnesh,Gadi, B. R.,&Rao, Satyawada Rama.(2021).Morphological and molecular markers based assessment of genetic diversity of a valuable endemic plant Lasiurus sindicus Henr. in the arid region of Rajasthan, India.ARID LAND RESEARCH AND MANAGEMENT.
MLA	Goswami, Bhuwnesh,et al."Morphological and molecular markers based assessment of genetic diversity of a valuable endemic plant Lasiurus sindicus Henr. in the arid region of Rajasthan, India".ARID LAND RESEARCH AND MANAGEMENT (2021).