干旱区生态环境知识资源中心(Arid): Potassium transporter HcKUP12 from Halostachys caspica improved transgenic Arabidopsis salt tolerance through maintaining potassium homeostasis

Arid

DOI	10.1007/s00468-021-02246-0
	Potassium transporter HcKUP12 from Halostachys caspica improved transgenic Arabidopsis salt tolerance through maintaining potassium homeostasis
	Zhang, Sheng; Zhang, Hua; You, Xilong; Yang, Zhongmin; Wang, Yan
通讯作者	Wang, Y (corresponding author), Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China.
来源期刊	TREES-STRUCTURE AND FUNCTION
ISSN	0931-1890
EISSN	1432-2285
出版年	2021-12
英文摘要	Potassium (K+) is an essential macronutrient for plant growth, development and resistance to osmotic stress. KT/HAK/KUP is the largest potassium transporter family, the members of which play crucial roles in K+ homeostasis and cell growth in various plant species. Desert halophytes need many strategies to survive in harsh environments, among which efficient absorption of K+ is an important mechanism. Here, we identified and studied HcKUP12 of the KT/HAK/KUP family from Halostachys caspica which is an important perennial halophyte of woody plants. Expression analysis showed that HcKUP12 has higher expression in assimilation branches (functioning as leaves due to leaf degeneration) than roots, and HcKUP12 was induced by salt, abscisic acid (ABA) and methyl viologen (MV) stress. Subcellular localization analysis indicated that HcKUP12 was targeted to the plasma membrane (PM). HcKUP12 gene function was evaluated using K+ uptake deficient yeast R5421 and Arabidopsis thaliana was evaluated. Transformation with HcKUP12 rescued the growth defect of mutant yeast strain R5421 at the low K+ concentration range between 0 and 50 mM. Overexpression of HcKUP12 in A. thaliana showed increased lateral roots at 100 mu M (- K+) supply condition and root length at 125 mM NaCl stress, respectively. Additionally, shoot growth, potassium content and K+/Na+ concentration ratio in the seedlings of OE lines were all enhanced resulting in tolerance to low potassium and salt stress. Taken together, these results demonstrate that HcKUP12 plays a major role in K+ acquisition when faced with externally low K+ and high Na+ in HcKUP12 OE plants.
英文关键词	Potassium transporter HcKUP12 Na+/K+ homeostasis Salt tolerance Halostachys caspica
类型	Article ; Early Access
语种	英语
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000725466900002
WOS关键词	K+ ACQUISITION ; ION HOMEOSTASIS ; GENE FAMILY ; RICE ; STRESS ; ABSORPTION ; MECHANISMS ; EXPRESSION ; CLONING ; GROWTH
WOS类目	Forestry
WOS研究方向	Forestry
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/374682
作者单位	[Zhang, Sheng; Zhang, Hua; You, Xilong; Yang, Zhongmin; Wang, Yan] Xinjiang Univ, Coll Life Sci & Technol, Xinjiang Key Lab Biol Resources & Genet Engn, Urumqi 830046, Peoples R China; [Zhang, Sheng] Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Sheng,Zhang, Hua,You, Xilong,et al. Potassium transporter HcKUP12 from Halostachys caspica improved transgenic Arabidopsis salt tolerance through maintaining potassium homeostasis[J],2021.
APA	Zhang, Sheng,Zhang, Hua,You, Xilong,Yang, Zhongmin,&Wang, Yan.(2021).Potassium transporter HcKUP12 from Halostachys caspica improved transgenic Arabidopsis salt tolerance through maintaining potassium homeostasis.TREES-STRUCTURE AND FUNCTION.
MLA	Zhang, Sheng,et al."Potassium transporter HcKUP12 from Halostachys caspica improved transgenic Arabidopsis salt tolerance through maintaining potassium homeostasis".TREES-STRUCTURE AND FUNCTION (2021).